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Yu, Z., Chen, Q., Chen, W., Zhang, X., Mei, F., Zhang, P., Zhao, M., Wang, X., Shi, N., Jackson, S. and Hong, Yiguo (2017) Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis. Journal of Integrative Plant Biology. ISSN 1672-9072 Online: 1744-7909
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Abstract
We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms. [Abstract copyright: This article is protected by copyright. All rights reserved.]
| Item Type: | Article |
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| Additional Information: | The full-text can be accessed via the Official URL. |
| Uncontrolled Discrete Keywords: | single-sgRNA, CRISPR/Cas9, AtRPL10A, AtRPL10B, AtRPL10C, |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
| SWORD Depositor: | Prof. Pub Router |
| Depositing User: | Yiguo Hong |
| Date Deposited: | 19 Jan 2018 10:19 |
| Last Modified: | 17 Jun 2020 17:20 |
| URI: | https://eprints.worc.ac.uk/id/eprint/6266 |
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