Lists
Lists
Apangu, Godfrey, Frisk, Carl 
ORCID: https://orcid.org/0000-0002-9722-2544, Petch, Geoffrey, Muggia, Lucia, Pallavicini, Alberto, Hanson, Mary and Skjøth, C. ORCID: https://orcid.org/0000-0001-5992-9568
(2022)
Environmental DNA reveals diversity and abundance of Alternaria species in neighbouring heterogeneous landscapes in Worcester, UK.
Aerobiologia.
ISSN Electronic: 1573-3025 Print: 0393-5965
![[thumbnail of Aerobiologia Accepted paper AERO-D-22-00009_R1.pdf]](https://eprints.worc.ac.uk/style/images/fileicons/text.png "Aerobiologia Accepted paper AERO-D-22-00009_R1.pdf") |
Text
Aerobiologia Accepted paper AERO-D-22-00009_R1.pdf - Accepted Version Restricted to Repository staff only Download (2MB) | Request a copy |
Preview |
Text (Open Access article)
s10453-022-09760-9.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Alternaria is a pathogenic and allergenic fungus affecting 400 plant species and 334 million people globally. This study aimed at assessing the diversity of Alternaria species in airborne samples collected from closely located (7 km apart) and heterogeneous sites (rural, urban and unmanaged grassland) in Worcester and Lakeside, the UK. A secondary objective was to examine how the ITS1 subregion varies from ITS2 in Alternaria species diversity and composition. Airborne spores were collected using Burkard 7-day and multi-vial Cyclone samplers for the period 5 July 2016–9 October 2019. Air samples from the Cyclone were amplified using the ITS1and ITS2 subregions and sequenced using Illumina MiSeq platform whereas those from the Burkard sampler were identified and quantified using optical microscopy. Optical microscopy and eDNA revealed a high abundance of Alternaria in the rural, urban and unmanaged sites. ITS1 and ITS2 detected five and seven different Alternaria species at the three sampling sites, respectively. A. dactylidicola, A. metachromatica and A. infectoria were the most abundant. The rural, urban and unmanaged grassland sites had similar diversity (PERMANOVA) of the species due to similarity in land use and proximity of the sites. Overall, the study showed that heterogeneous and neighbouring sites with similar land uses can have similar Alternaria species. It also demonstrated that an eDNA approach can complement the classical optical microscopy method in providing more precise information on fungal species diversity in an environment for targeted management. Similar studies can be replicated for other allergenic and pathogenic fungi.
| Item Type: | Article |
|---|---|
| Additional Information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| Uncontrolled Discrete Keywords: | Metabarcoding, Microscopy, Fungi, ITS1, ITS2, Unmanaged grassland |
| Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
| Copyright Info: | © The Author(s) 2022 |
| Depositing User: | Godfrey Apangu |
| Date Deposited: | 18 Oct 2022 10:14 |
| Last Modified: | 07 Jun 2023 07:34 |
| URI: | https://eprints.worc.ac.uk/id/eprint/12553 |
Actions (login required)
 |
View Item |
