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A Comparison of Peronospora parasitica (Downy mildew) Isolates from Arabidopsis thaliana and Brassica oleracea Using Amplified Fragment Length Polymorphism and Internal Transcribed Spacer 1 Sequence Analyses

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Rehmany, A.P., Lynn, J.R., Tör, M. ORCID: https://orcid.org/0000-0002-4416-5048, Holub, E.B. and Beynon, J. (2000) A Comparison of Peronospora parasitica (Downy mildew) Isolates from Arabidopsis thaliana and Brassica oleracea Using Amplified Fragment Length Polymorphism and Internal Transcribed Spacer 1 Sequence Analyses. Fungal Genetics and Biology, 30 (2). pp. 95-103.

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Official URL: http://tinyurl.com/5tf7rfu

Abstract

Amplified fragment length polymorphism (AFLP) fingerprints and internal transcribed spacer 1 (ITS1) sequences from 27 Peronospora parasitica isolates (collected from Arabidopsis thaliana or Brassica oleracea), 5 Albugo candida isolates (from the same hosts and from Capsella bursa-pastoris), and 1 Bremia lactucae isolate (from Lactuca sativa) were compared. The AFLP analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. The only exception was a group consisting of A. candida isolates from both B. oleracea and C. bursa-pastoris. ITS1 sequence analysis divided the isolates into the same five groups, demonstrated the divergence between P. parasitica isolates from A. thaliana and B. oleracea, and, using previously published ITS1 sequences, clearly showed the relationship between A. candida isolates from different hosts.

Item Type:	Article
Additional Information:	The electronic full-text cannot be supplied for this item. Please check availability with your local library or Interlibrary Requests Service. Originally deposited as joint division with National Pollen and Aerobiology Research Unit (NPARU)
Uncontrolled Discrete Keywords:	phylogenetic tree, Peronosporales, oomycete, Downey Mildew, Arabidopsis
Subjects:	Q Science > QK Botany Q Science > QH Natural history > QH426 Genetics Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
Depositing User:	Janet Davidson
Date Deposited:	19 Jan 2011 19:55
Last Modified:	17 Jun 2020 16:54
URI:	https://eprints.worc.ac.uk/id/eprint/1172

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