Worcester Research and Publications

SEARCH EPRINTS:

USER PANEL:

Registered Users - Log in using your University of Worcester ID and Password to submit items to the repository.

ABOUT THE COLLECTION:

WRaP is a collection of research papers and university publications. It presents the academic and creative work of the university. You are welcome to look for and obtain items of interest and make contact with the authors and creators.

CONTACT DETAILS:

All correspondence about WRaP should be sent to the Repository Manager.

Factors Influencing Barley Stripe Mosaic Virus-Mediated Gene Silencing in Wheat

Lists

Tools

Cakir, C. and Tör, M. ORCID: https://orcid.org/0000-0002-4416-5048 (2010) Factors Influencing Barley Stripe Mosaic Virus-Mediated Gene Silencing in Wheat. Physiological and Molecular Plant Biology, 74 (3-4). pp. 246-253. ISSN 0885-5765

Full text not available from this repository. (Request a copy)

Official URL: http://ac.els-cdn.com/S0885576510000196/1-s2.0-S08...

Abstract

Virus induced gene silencing (VIGS) is a technology that has been used primarily to target the virus genome in infected plants. However, if the virus genome carries inserts derived from the host plant, the system could be employed to target the mRNAs corresponding to the host gene. Barley Stripe Mosaic Virus (BSMV), a disease of various cereals including barley and wheat, has been one of the successfully used viral RNA silencing tools in monocotyledonous plants for the last decade. In this study, we investigated several factors that play a significant role in VIGS. We tested the efficiency of silencing two genes simultaneously using the BSMV-induced gene silencing (IGS) system in detail. We found that two genes could be silenced simultaneously using BSMV-IGS. However, the silencing efficiency was found to be influenced by several factors including stability of the insert, temperature, and the accumulation of small viral RNAs from BSMV. The effect of these factors on VIGS system has been discussed.

Item Type:	Article
Additional Information:	The full-text can be accessed via the official URL. Originally deposited as a joint division with National Pollen and Aerobiology Research Unit (NPARU)
Uncontrolled Discrete Keywords:	BSMV, insert stability, simultaneous gene silencing, viral small RNAs, virus induced gene silencing, wheat, plant diseases, plant-pathogen relationships, molecular biology, plant pathology
Subjects:	Q Science > QK Botany Q Science > QR Microbiology > QR355 Virology Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology Q Science > QR Microbiology
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:	Publisher
Depositing User:	Janet Davidson
Date Deposited:	19 Jan 2011 19:24
Last Modified:	17 Jun 2020 16:54
URI:	https://eprints.worc.ac.uk/id/eprint/1157

Actions (login required)

View Item

Altmetric

CORE (COnnecting REpositories)

© University of Worcester Henwick Grove, WR2 6AJ Tel: 01905 855000 | Materials in WRaP are protected by copyright and other intellectual property rights. By using WRaP you agree to abide by UK copyright laws.

Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.