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Leukocyte Hsp72 mRNA Transcription Does Not Differ Between Males and Females During Heat Acclimation

Mee, Jessica A., Gibson, O.R., Tuttle, J.A., Watt, P.W., Doust, J. and Maxwell, N.S. (2016) Leukocyte Hsp72 mRNA Transcription Does Not Differ Between Males and Females During Heat Acclimation. Temperature, 3 (4). pp. 549-556. ISSN 2332-8940

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Abstract

Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription. Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR). Downloaded by [ASPIRE] at 22:18 27 July 2016 2 Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold). Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.

Item Type: Article
Additional Information:

This is an Accepted Manuscript of an article published by Taylor & Francis in Temperature on 27/07/2016, available online: http://www.tandfonline.com/10.1080/23328940.2016.1214336.

Staff and students at the University of Worcester can access the full-text of the published version via the UW online library search. External users should check availability with their local library or Interlibrary Requests Service.

Uncontrolled Keywords: controlled hyperthermia, females, heat shock protein, males, thermotolerance
Subjects: Q Science > QP Physiology
Divisions: Academic Departments > Institute of Sport and Exercise Science
Related URLs:
Depositing User: Jessica Mee
Date Deposited: 06 Jun 2019 11:58
Last Modified: 06 Jun 2019 11:58
URI: https://eprints.worc.ac.uk/id/eprint/8121

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