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Identifying Molecular Markers Suitable For Frl Selection in Tomato Breeding

Zübeyir, D., Kahveci, E., Hong, Yiguo, Studholme, D.J. and Tör, M. (2018) Identifying Molecular Markers Suitable For Frl Selection in Tomato Breeding. Theoretical and Applied Genetics. ISSN 0040-5752 Online: 1432-2242

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Abstract

Modern plant breeding heavily relies on the use of molecular markers. In recent years, next generation sequencing (NGS) emerged as a powerful technology to discover DNA sequence polymorphisms and generate molecular markers very rapidly and cost effectively, accelerating the plant breeding programmes. A single dominant locus, Frl, in tomato provides resistance to the fungal pathogen Fusarium oxysporum f. sp. radicis-lycopersici (FORL), causative agent of Fusarium crown and root rot. In this study, we describe the generation of molecular markers associated with the Frl locus. An F2 mapping population between an FORL resistant and a susceptible cultivar was generated. NGS technology was then used to sequence the genomes of a susceptible and a resistant parent as well the genomes of bulked resistant and susceptible F2 lines. We zoomed into the Frl locus and mapped the locus to a 900 kb interval on chromosome 9. Polymorphic single-nucleotide polymorphisms (SNPs) within the interval were identified and markers co-segregating with the resistant phenotype were generated. Some of these markers were tested successfully with commercial tomato varieties indicating that they can be used for marker-assisted selection in large-scale breeding programmes.

Item Type: Article
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The online version of this article ( https://doi.org/10.1007/s00122-018-3136-0) contains supplementary material, which is available to authorized users.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Uncontrolled Keywords: FORL resistance locus (Frl), plant breeding, tomato breeding, plant genetics, molecular markers, NGS technology
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Academic Departments > Institute of Science and the Environment
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Copyright Info: Open Access
Depositing User: Mahmut Tor
Date Deposited: 09 Jul 2018 12:22
Last Modified: 09 Jul 2018 12:22
URI: https://eprints.worc.ac.uk/id/eprint/6881

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