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A Suppressor/Avirulence Gene Combination in Hyaloperonospora Arabidopsidis Determines Race Specificity in Arabidopsis Thaliana

Woods-Tör, Alison and Studholme, D.J. and Cevik, V. and Telli, O. and Holub, E.B. and Tör, M. (2018) A Suppressor/Avirulence Gene Combination in Hyaloperonospora Arabidopsidis Determines Race Specificity in Arabidopsis Thaliana. Frontiers in Plant Science, 9 (265). ISSN 1664-462X

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Abstract

The pathosystem of Arabidopsis thaliana and diploid biotrophic oomycete Hyaloperonospora arabidopsidis (Hpa) has been a model for investigating the molecular basis of Flor’s gene-for-gene hypothesis. The isolates Hpa-Noks1 and Hpa-Cala2 are virulent on Arabidopsis accession RMX-A02 whilst an F1 generated from a cross between these two isolates was avirulent. The F2 progeny segregated 3,1 (avirulent, virulent), indicating a single major effect AVR locus in this pathogen. SNP-based linkage mapping confirmed a single AVR locus within a 14 kb map interval containing two genes encoding putative effectors. The Hpa-Cala2 allele of one gene, designated H. arabidopsidis cryptic1 (HAC1), encodes a protein with a signal peptide and an RxLR/dEER motif, and triggers a defense response in RMX-A02. The second gene is heterozygous in Hpa-Cala2. One allele, designated Suppressor of HAC1Cala2 (S-HAC1Cala2) encodes a protein with a signal peptide and a dKEE motif with no RxLR motif; the other allele (s-hac1 Cala2) encodes a protein with a signal peptide, a dEEE motif and is divergent in sequence from the S-HAC1Cala2 allele. In selfed progeny from Hpa-Cala2, dominant S-HAC1Cala2 allele carrying progeny correlates with virulence in RMX-A02, whereas homozygous recessive s-hac1Cala2 carrying progeny were avirulent. Genetic investigations suggested other heterozygous suppressor loci might exist in the Hpa-Cala2 genome.

Item Type: Article
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Uncontrolled Keywords: effector, avirulence inhibitor, suppressor, oomycete, Arabidopsis, downy mildew, heterozygosity
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
Divisions: Academic Departments > Institute of Science and the Environment
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Copyright Info: Open Access
Depositing User: Karol Kosinski
Date Deposited: 07 Mar 2018 09:39
Last Modified: 07 Mar 2018 09:39
URI: https://eprints.worc.ac.uk/id/eprint/6454

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