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Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis

Yu, Z., Chen, Q., Chen, W., Zhang, X., Mei, F., Zhang, P., Zhao, M., Wang, X., Shi, N., Jackson, S. and Hong, Yiguo (2017) Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis. Journal of Integrative Plant Biology. ISSN 1672-9072 Online: 1744-7909

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Abstract

We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms. [Abstract copyright: This article is protected by copyright. All rights reserved.]

Item Type: Article
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Uncontrolled Keywords: single-sgRNA, CRISPR/Cas9, AtRPL10A, AtRPL10B, AtRPL10C,
Subjects: Q Science > Q Science (General)
Divisions: Academic Departments > Institute of Science and the Environment
SWORD Depositor: Prof. Pub Router
Depositing User: Yiguo Hong
Date Deposited: 19 Jan 2018 10:19
Last Modified: 11 Dec 2018 01:00
URI: https://eprints.worc.ac.uk/id/eprint/6266

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