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CD200 Inhibits Memory Th1 Cell Function in Acute Myeloid Leukaemia (AML)

Coles, Steven and Man, S. and Hills, R.K. and Wang, E.C.Y. and Burnett, A.K. and Darley, R.L. and Tonks, A. (2011) CD200 Inhibits Memory Th1 Cell Function in Acute Myeloid Leukaemia (AML). In: Annual Congress of the British Society for Immunology, 5th - 8th December 2011, Liverpool, UK. (Unpublished)

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Abstract

CD200 is a cell-surface glycoprotein that is normally expressed in tissues of the immune system, where its role is to protect immune privileged sites. We previously established CD200 to be frequently over-expressed and associated with poor AML patient outcome. In this study, we investigated the possibility that CD200 expression may mediate suppression of T-cell function in this disease. Using multiparameter flow cytometry, we compared PMA/ionomycin stimulated CD8+ T-cell cytotoxic potential (CD107a expression) and the frequency of intracellular TNFa, IL-2 and IFNc producing CD4+/CD8+ memory T-cells between CD200hi and CD200lo patients. We demonstrated that both the magnitude of the CD8+ memory cytotoxic T-cell response and the Th1 cytokine producing CD4+ memory helper T-cells was significantly inhibited in CD200hi AML patients (P < 0.05). Further, using ELISPOT assays to measure IFNg release we showed that the Th1 memory response to common viral antigens was significantly reduced by 75% in CD200hi versus CD200lo AML patients(P < 0.05). Recovery of IFNc release in response to recall antigens was observed in CD4+ memory T-cells incubated with a blocking antibody to CD200R. In conclusion, this study shows a correlation between T-cell dysfunction and expression of CD200 which suggests targeting this axis could be therapeutically beneficial for AML CD200hi patients.

Item Type: Conference or Workshop Item (Poster)
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Uncontrolled Keywords: CD200 expression, AML patients, Acute Myeloid Leukaemia, T-cell function
Subjects: R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Academic Departments > Institute of Science and the Environment
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Depositing User: Steven Coles
Date Deposited: 15 Sep 2017 08:47
Last Modified: 15 Sep 2017 10:51
URI: https://eprints.worc.ac.uk/id/eprint/5882

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