WRaP - Worcester Research and Publications

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Biography

Expertise:

Immunology, Monoclonal antibodies, Pathology, Biomedical detection systems

Job title:

Research Scientist

Biography:

I am the monoclonal antibody unit manager at the University of Worcester. I also works within a team investigating the development and use of immunological assay systems to study both plant pathogen incidence within horticultural cropping systems, as well as disease detection in the Biomedical field. Once developed the antibodies can be applied to a number of detection platforms such as enzyme-linked immunosorbent assay, (ELISA), Immunofluorescence, Western blot and lateral flow device technology (LFDs).

Qualifications:

BSc, MSc, AFHEA

My Latest As Author

Doughty, Angela, Keane, Gary, Wadley, Alex, Mahoney, Berenice ORCID: https://orcid.org/0000-0002-7414-8185, Bueno, Allain ORCID: https://orcid.org/0000-0002-9456-8558 and Coles, Steven ORCID: https://orcid.org/0000-0002-1109-6971 (2024) Plasma concentrations of thioredoxin, thioredoxin reductase and peroxiredoxin-4 can identify high risk patients and predict outcome in patients with acute coronary syndrome: a clinical observation. International Journal of Cardiology. pp. 1-36. ISSN 0167-5273 (print) 1874-1754 (web)
Wadley, Alex, Cullen, Tom, Vautrinot, Jordan, Keane, Gary, Bishop, N.C. and Coles, Steven ORCID: https://orcid.org/0000-0002-1109-6971 (2020) High intensity interval exercise increases the frequency of peripheral PD-1+ CD8+ central memory T-cells and soluble PD-L1 in humans. Brain Behaviour and Immunity: Health, 3. Article no. 100049. ISSN 2666-3546
Steward, Charles, Zhou, Y., Keane, Gary, Cook, Matthew ORCID: https://orcid.org/0000-0002-6293-7566, Liu, Y. and Cullen, Tom (2019) One week of magnesium supplementation lowers IL-6, muscle soreness and increases 1 post exercise blood glucose in response to downhill running. European Journal of Applied Physiology, 119 (11-12). pp. 2617-2627. ISSN Print: 1439-6319 Online: 1439-6327
Wadley, A., Keane, Gary, Cullen, Tom, James, L., Vautrinot, Jordon, Davies, Matthew, Hussey, B., Hunter, D.J., Mastana, S., Holliday, A., Petersen, SV., Bishop, N.C., Lindley, M.R. and Coles, Steven ORCID: https://orcid.org/0000-0002-1109-6971 (2019) Characterisation of Extracellular Redox Enzyme Concentrations in Response to Exercise in Humans. Journal of Applied Physiology, 127 (3). pp. 858-866. ISSN Print: 8750-7587 Online: 1522-1601
Pettitt, Timothy, Keane, Gary and John, Simon (2019) Atypical Late Blight Symptoms Following First Recorded Infections by Phytophthora Infestans Genotype EU_39_A1 in UK Vine Tomatoes. New Disease Reports, 39. p. 16. ISSN 2044-0588
Wakeham, Alison, Pettitt, Timothy, Keane, Gary, John, Simon, Lewis, M. and Edwards, Emma (2019) CP 136: Development and Testing of Single and Multiple Diagnostic Devices For Rapid and Precise Early Detection of Oomycete Root and Collar Rot Pathogens for Disease Avoidance, Management and Control. Annual Report November 2015. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Wadley, A., Hole, P., Tonks, A., Darley, R.L., Omidvar, N., Morgan, R., Keane, Gary and Coles, Steven ORCID: https://orcid.org/0000-0002-1109-6971 (2018) Intracellular Thiol Oxidation Is Linked with Loss of Δψm and Disease Progression in Acute Promyelocytic Leukaemia. In: 60th ASH Annual Meeting & Exposition, 1st - 4th December 2018, San Diego Convention Center, CA, USA. (Unpublished)
Wakeham, Alison, Keane, Gary, John, Simon, Adams-Groom, Beverley ORCID: https://orcid.org/0000-0002-1097-8876 and Kennedy, Roy (2018) CP 137 - Cucumber: Development and Testing of a Lateral Flow Device For Both Gummy Stem Blight and Powdery Mildew in Bio-aerosols During Cucurbit Production. Final Report. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Keane, Gary (2018) Purification of a Monoclonal Antibody to N. ditissima Cell Culture Medium. Project Report. East Malling Research.
Keane, Gary (2018) Production of 500 mls of a Monoclonal Antibody to N. ditissima in Cell Culture Medium. Project Report. East Malling Research.
Kennedy, Roy, Lewis, Mary, Petch, Geoffrey, Warren, A., Edwards, Emma, Keane, Gary, Proctor, Maureen, John, Simon and Wakeham, Alison (2017) Further Development of “in field” Tests for Resting Spores of Clubroot and the Development of Control Based on Detection. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Wakeham, Alison, Keane, Gary and Kennedy, R. (2016) Field Evaluation of a Competitive Lateral-flow Assay for Detection of Alternaria brassicae in Vegetable Brassica Crops. Plant Disease, 100 (9). pp. 1831-1839. ISSN 0191-2917
Wakeham, Alison, Keane, Gary, John, Simon and Petch, Geoffrey (2016) CP 099c - Evaluation of an Integrated Disease Management System to Ascribe Risk of Downy Mildew Disease on Commercial Salad and Bulb Onion Crops in the UK. Final Report. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Wakeham, Alison, Keane, Gary, John, Simon, Lewis, M. and Petch, Geoffrey (2016) CP - 137 Development and Testing of a Lateral Flow Device For Both Gummy Stem Blight and Powdery Mildew in Bio-aerosols During Cubit Production Annual Report, November 2015. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Keane, Gary and John, Simon (2015) Demonstration of Onion Downy Mildew Lateral Flow Devices. [Show/Exhibition]
Wakeham, Alison, Lewis, M., Keane, Gary, Edwards, Emma, Petch, Geoffrey and John, Simon (2015) CP 099a - Validation of the Clubroot Lateral Flow in UK Commercial Brassica Cropping Systems. Final Report, 2015. Project Report. Agriculture and Horticulture Development Board, Warwickshire. UK.
Wakeham, Alison, Keane, Gary, John, Simon and Petch, Geoffrey (2015) CP-099 Diagnostics: Validation of the Lateral Flow Detection Devices for the Light Leaf Spot and Powdery Mildew Vegetable Brassica Pathogens and Testing of White Blister Detection Test Prototypes. Final Report, August 2015. Project Report. Agriculture and Horticulture Development Board, Warwickshire, UK.
Keane, Gary (2015) Maintenance, Storage, Purification, and Preliminary Kinetic Studies of 3 Monoclonal Cell Lines (Provided). Project Report. UNSPECIFIED.
Wakeham, Alison, Lewis, Mary and Keane, Gary (2014) CP 099a - Validation of the Clubroot Lateral Flow in UK Commercial Brassica Cropping Systems. Annual Report, April 2014. Project Report. The Horticultural Development Company, Kenilworth.
Kennedy, Roy, Wakeham, Alison, Lewis, Mary, Keane, Gary, Petch, Geoffrey, Proctor, Maureen and John, Simon (2013) FV 349 - Brassicas: Further Development of "in Field" Tests for Resting Spores of Clubroot and the Development of Clubroot Control Based on Detection. Project Report. HDC, Kenilworth.
Wakeham, Alison, Keane, Gary, Proctor, Maureen and Petch, Geoffrey (2013) CP 099 - Diagnostics: Validation of the Lateral Flow Detection Devices for the Light Leaf Spot and Powdery Mildew Vegetable Brassica Pathogens and Testing of White Blister Detection Test Prototypes. Project Report. The Horticultural Development Company, Kenilworth.
Kennedy, Roy, Wakeham, Alison, Keane, Gary, Petch, Geoffrey, Proctor, Maureen and John, Simon (2012) FV 356 - Onions: Further Development and Calibration of Detection Tests for Conidia of Onion Downy Mildew in Combination with the Morph Forecast Model MILIONCAST. Project Report. The Horticultural Development Company, Kenilworth.
Wakeham, Alison, Keane, Gary, Proctor, Maureen and Kennedy, Roy (2012) Monitoring Infection Risk for Air and Soil Borne Fungal Plant Pathogens using Antibody and DNA Techniques and Mathematical Models describing Environmental Parameters. In: Microbes in Applied Research: Current Advances and Challenges. World Scientific Publishing Co. Pte. Ltd, Singapore, pp. 152-156. ISBN Paperback: 981-4405-03-5 Ebook: 981-4405-05-8
Wakeham, Alison and Keane, Gary (2011) Horticulture Australia Limited (HAL) – VG07070: Benchmarking Predictive Models, Nutrients and Irrigation for Management of White Blister. Subcontract Final Report. Project Report. Horticulture Australia Limited, Sydney, Australia. (Unpublished)

My Most Viewed As Author

#	Item title
1.	Intracellular Thiol Oxidation Is Linked with Loss of Δψm and Disease Progression in Acute Promyelocytic Leukaemia	863	3
2.	Characterisation of Extracellular Redox Enzyme Concentrations in Response to Exercise in Humans	356	63
3.	Horticulture Australia Limited (HAL) – VG07070: Benchmarking Predictive Models, Nutrients and Irrigation for Management of White Blister. Subcontract Final Report	291	8
4.	One week of magnesium supplementation lowers IL-6, muscle soreness and increases 1 post exercise blood glucose in response to downhill running	288	815
5.	Demonstration of Onion Downy Mildew Lateral Flow Devices	265
6.	Further Development of “in field” Tests for Resting Spores of Clubroot and the Development of Control Based on Detection	255	5
7.	Monitoring Infection Risk for Air and Soil Borne Fungal Plant Pathogens using Antibody and DNA Techniques and Mathematical Models describing Environmental Parameters	255	3
8.	CP 099 - Diagnostics: Validation of the Lateral Flow Detection Devices for the Light Leaf Spot and Powdery Mildew Vegetable Brassica Pathogens and Testing of White Blister Detection Test Prototypes	225
9.	FV 356 - Onions: Further Development and Calibration of Detection Tests for Conidia of Onion Downy Mildew in Combination with the Morph Forecast Model MILIONCAST	225
10.	CP 137 - Cucumber: Development and Testing of a Lateral Flow Device For Both Gummy Stem Blight and Powdery Mildew in Bio-aerosols During Cucurbit Production. Final Report	210
11.	CP 099a - Validation of the Clubroot Lateral Flow in UK Commercial Brassica Cropping Systems. Annual Report, April 2014	202
12.	Purification of a Monoclonal Antibody to N. ditissima Cell Culture Medium	200
13.	CP - 137 Development and Testing of a Lateral Flow Device For Both Gummy Stem Blight and Powdery Mildew in Bio-aerosols During Cubit Production Annual Report, November 2015	196
14.	High intensity interval exercise increases the frequency of peripheral PD-1+ CD8+ central memory T-cells and soluble PD-L1 in humans	193	81
15.	Maintenance, Storage, Purification, and Preliminary Kinetic Studies of 3 Monoclonal Cell Lines (Provided)	191
16.	Field Evaluation of a Competitive Lateral-flow Assay for Detection of Alternaria brassicae in Vegetable Brassica Crops	187	126
17.	FV 349 - Brassicas: Further Development of "in Field" Tests for Resting Spores of Clubroot and the Development of Clubroot Control Based on Detection	180
18.	CP 099c - Evaluation of an Integrated Disease Management System to Ascribe Risk of Downy Mildew Disease on Commercial Salad and Bulb Onion Crops in the UK. Final Report	170
19.	Plasma concentrations of thioredoxin, thioredoxin reductase and peroxiredoxin-4 can identify high risk patients and predict outcome in patients with acute coronary syndrome: a clinical observation	163	37
20.	CP-099 Diagnostics: Validation of the Lateral Flow Detection Devices for the Light Leaf Spot and Powdery Mildew Vegetable Brassica Pathogens and Testing of White Blister Detection Test Prototypes. Final Report, August 2015	159
21.	Production of 500 mls of a Monoclonal Antibody to N. ditissima in Cell Culture Medium	154
22.	CP 099a - Validation of the Clubroot Lateral Flow in UK Commercial Brassica Cropping Systems. Final Report, 2015	147
23.	CP 136: Development and Testing of Single and Multiple Diagnostic Devices For Rapid and Precise Early Detection of Oomycete Root and Collar Rot Pathogens for Disease Avoidance, Management and Control. Annual Report November 2015	135
24.	Atypical Late Blight Symptoms Following First Recorded Infections by Phytophthora Infestans Genotype EU_39_A1 in UK Vine Tomatoes	131

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Gary Keane