University of Worcester Worcester Research and Publications

An RNAi suppressor activates in planta virus–mediated gene editing

Zhang, X., Kang, L., Zhang, Q., Meng, Q., Pan, Y., Yu, Z., Shi, N., Jackson, S., Zhang, X., Wang, H., Tör, M. ORCID: and Hong, Yiguo (2020) An RNAi suppressor activates in planta virus–mediated gene editing. Functional & Integrative Genomics, 20. pp. 471-477. ISSN Print: 1438-793X Online: 1438-7948

AAM-with-cover-9008-Hong-Tor-RNAi-suppressor-activates-in-planta-virus-mediated-gene-editing.pdf - Accepted Version

Download (693kB) | Preview


RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.

Item Type: Article
Additional Information:

Staff and students at the University of Worcester can access the full-text of the online published article via the official URL. External users should check availability with their local library or Interlibrary Requests Service.

Uncontrolled Discrete Keywords: CRISPR/Cas9, FoMV, VmGE, RNAi suppressor, SERG
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:
Depositing User: Mahmut Tor
Date Deposited: 19 Dec 2019 11:41
Last Modified: 20 Oct 2022 14:59

Actions (login required)

View Item View Item
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.