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Zhang, X., Kang, L., Zhang, Q., Meng, Q., Pan, Y., Yu, Z., Shi, N., Jackson, S., Zhang, X., Wang, H., Tör, M. 
ORCID: https://orcid.org/0000-0002-4416-5048 and Hong, Yiguo
(2020)
An RNAi suppressor activates in planta virus–mediated gene editing.
Functional & Integrative Genomics, 20.
pp. 471-477.
ISSN Print: 1438-793X Online: 1438-7948
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AAM-with-cover-9008-Hong-Tor-RNAi-suppressor-activates-in-planta-virus-mediated-gene-editing.pdf - Accepted Version Download (693kB) | Preview |
Abstract
RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.
| Item Type: | Article |
|---|---|
| Additional Information: | Staff and students at the University of Worcester can access the full-text of the online published article via the official URL. External users should check availability with their local library or Interlibrary Requests Service. |
| Uncontrolled Discrete Keywords: | CRISPR/Cas9, FoMV, VmGE, RNAi suppressor, SERG |
| Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
| Related URLs: | |
| Depositing User: | Mahmut Tor |
| Date Deposited: | 19 Dec 2019 11:41 |
| Last Modified: | 20 Oct 2022 14:59 |
| URI: | https://eprints.worc.ac.uk/id/eprint/9008 |
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