The Direct Inhibition of MMP–2 and MMP–9 by an Enzyme Alginogel: A Possible Mechanism of Healing Support for Venous Leg Ulcers

Grzela, T., Niderla-Bielinska, J., Litwiniuk, M. and White, Richard (2014) The Direct Inhibition of MMP–2 and MMP–9 by an Enzyme Alginogel: A Possible Mechanism of Healing Support for Venous Leg Ulcers. Journal of Wound Care, 23 (5). pp. 278-285. ISSN 0969-0700

Abstract

Flaminal Forte is an enzyme alginogel,whose activity depends on the absorption and binding of matrix metalloproteinases (MMPs), which are known to play a crucial role in delayed wound healing. The aim of the study was to evaluate the influence of Flaminal on MMP-2/-9 activity in ulcer exudate, ex vivo. Eight patients with bilateral venous leg ulcers were treated for 4 weeks with Flaminal Forte covered by hydrocolloid ('F' wounds), or with hydrocolloid alone ('H' wounds) as a reference control. Clinical assessment did not reveal any differences between F and H wounds regarding surface reduction and general wound condition. Nevertheless, although non-significant, there was a visible difference in peri-wound skin appearance in F wounds, as compared to H wounds. The wound exudate contained high MMP-2/-9 levels, which gradually decreased as wounds healed. The attenuation of MMPs was stronger in F than in H exudate, however, in standard zymography this difference appeared non-significant. Realtime zymography revealed that Flaminal mediated a powerful direct inhibition of gelatinolytic activity of wound exudate and of recombinant MMP-2/-9 in vitro.

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