University of Worcester Worcester Research and Publications

Expression of Arabidopsis WEE1 in Tobacco Induces Unexpected Morphological and Developmental Changes

Siciliano, Ilario, Lentz Grønlund, A., Ševčíková, H., Spadafora, Natasha D., Rafiei, G., Francis, D., Herbert, Rob, Bitonti, M.B., Rogers, H.J. and Lipavská, H. (2019) Expression of Arabidopsis WEE1 in Tobacco Induces Unexpected Morphological and Developmental Changes. Scientific Reports, 9 (1). Article no. 8695. ISSN Online: 2045-2322

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WEE1 regulates the cell cycle by inactivating cyclin dependent protein kinases (CDKs) via phosphorylation. In yeast and animal cells, CDC25 phosphatase dephosphorylates the CDK releasing cells into mitosis, but in plants, its role is less clear. Expression of fission yeast CDC25 (Spcdc25) in tobacco results in small cell size, premature flowering and increased shoot morphogenetic capacity in culture. When Arath;WEE1 is over-expressed in Arabidopsis, root apical meristem cell size increases, and morphogenetic capacity of cultured hypocotyls is reduced. However expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced. This phenotype is similar to expression of Spcdc25 and is consistent with a dominant negative effect on WEE1 action. Consistent with this putative mechanism, WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis. The phenotype is not due to sense-mediated silencing of WEE1, as overall levels of WEE1 transcript were not reduced in BY2 lines expressing Arath;WEE1. However the pattern of native WEE1 transcript accumulation through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native WEE1 transcription.

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Uncontrolled Discrete Keywords: cell size, flowering, morphogenesis, plant cell cycle, tobacco BY2 cells, 49 transcriptional control, WEE1, SERG
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
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Copyright Info: Open access article
Depositing User: Rob Herbert
Date Deposited: 19 Jun 2019 10:12
Last Modified: 31 Jul 2020 14:18

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