Worcester Research and Publications

SEARCH EPRINTS:

USER PANEL:

Registered Users - Log in using your University of Worcester ID and Password to submit items to the repository.

ABOUT THE COLLECTION:

WRaP is a collection of research papers and university publications. It presents the academic and creative work of the university. You are welcome to look for and obtain items of interest and make contact with the authors and creators.

CONTACT DETAILS:

All correspondence about WRaP should be sent to the Repository Manager.

Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis

Lists

Tools

Yu, Z., Chen, Q., Chen, W., Zhang, X., Mei, F., Zhang, P., Zhao, M., Wang, X., Shi, N., Jackson, S. and Hong, Yiguo (2017) Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis. Journal of Integrative Plant Biology. ISSN 1672-9072 Online: 1744-7909

[thumbnail of MS_Text_Hong_JIPBrev-FV.pdf]

Preview

Text
MS_Text_Hong_JIPBrev-FV.pdf - Accepted Version
Download (120kB) | Preview

Official URL: http://onlinelibrary.wiley.com/doi/10.1111/jipb.12...

Abstract

We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms. [Abstract copyright: This article is protected by copyright. All rights reserved.]

Item Type:	Article
Additional Information:	The full-text can be accessed via the Official URL.
Uncontrolled Discrete Keywords:	single-sgRNA, CRISPR/Cas9, AtRPL10A, AtRPL10B, AtRPL10C,
Subjects:	Q Science > Q Science (General)
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
SWORD Depositor:	Prof. Pub Router
Depositing User:	Yiguo Hong
Date Deposited:	19 Jan 2018 10:19
Last Modified:	17 Jun 2020 17:20
URI:	https://eprints.worc.ac.uk/id/eprint/6266

Actions (login required)

View Item

Altmetric

CORE (COnnecting REpositories)

© University of Worcester Henwick Grove, WR2 6AJ Tel: 01905 855000 | Materials in WRaP are protected by copyright and other intellectual property rights. By using WRaP you agree to abide by UK copyright laws.

Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.