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A Novel Method for Detection of Viable Zoospores of Pythium in Irrigation Water

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Wakeham, Alison, Pettitt, Timothy and White, J.G. (1997) A Novel Method for Detection of Viable Zoospores of Pythium in Irrigation Water. Annals of Applied Biology, 131 (3). pp. 427-435. ISSN Online: 1744-7348

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1744-...

Abstract

A membrane filtration test has been developed for the detection of viable zoospores of Pythium species. Zoospore suspensions were filtered through 5 (m nitrocellulose membranes and the membranes incubated overnight in 0.07 M glucose, rifamycin (30 mg/litre)and pimaricin (100 mg/litre). Zoospore germlings were detected using a polyclonal antiserum, raised to mycelial surface washings of five Pythium spp., and visualised with Sigma fast red. The assay gave positive results for all Pythium spp. tested and also to zoospores of Phytophthora cryptogea. Of 10 fungal species isolated from commercial irrigation water, two were detected by the polyclonal antiserum in ELISA tests but only one produced detectable zoospore germlings. The latter isolate was later identified as a Pythium sp. Irrigation water samples collected from commercial UK nurseries yielded zoospores of both Pythium and Phytophthora spp. which, using the assay, were positively identified. Results indicated greater sensitivity than was seen with conventional plating methods. This is a test which could be adapted for on-site use in commercial nurseries.

Item Type:	Article
Additional Information:	The full-text of this article is available on Level 4 at The Hive in: Wakeham, Alison. The development and application of immunological tests within horticultural crop disease management systems. University of Worcester, PhD by Portfolio, 2014. Staff and students at the University of Worcester can access the full-text via Library Search. External users should check availability with their local library or Interlibrary Requests Service. Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)
Uncontrolled Discrete Keywords:	Pythium, polyclonal antibody, serological assay, zoospore, irrigation water
Subjects:	Q Science > QK Botany Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology S Agriculture > SB Plant culture
Divisions:	College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:	Publisher http://librarysearch.worc.ac.uk/
Depositing User:	Alison Wakeham
Date Deposited:	19 May 2015 17:31
Last Modified:	17 Jun 2020 17:06
URI:	https://eprints.worc.ac.uk/id/eprint/3701

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