Burns, D., Tierney, R., Shannon-Lowe, C., Croudace, Joanne, Inman, C., Abbotts, B., Nagra, S., Fox, C., Chaganti, S., Craddock, C., Moss, P., Rickinson, A., Rowe, M. and Bell, A. (2015) Memory B-cell reconstitution following allogeneic hematopoietic stem cell transplantation is an EBV-associated transformation event. Blood, 126 (25). pp. 2665-2675. ISSN Print: 0006-4971 Online: 1528-0020
Full text not available from this repository. (Request a copy)Abstract
Allogeneic stem cell transplantation (allo-HSCT) provides a unique opportunity to track Epstein-Barr virus (EBV) infection in the context of the reconstituting B-cell system. Although many allo-HSCT recipients maintain low or undetectable levels of EBV DNA posttransplant, a significant proportion exhibit elevated and rapidly increasing EBV loads which, if left untreated, may lead to potentially fatal EBV-associated posttransplant lymphoproliferative disease. Intriguingly, this high-level EBV reactivation typically arises in the first 3 months posttransplant, at a time when the peripheral blood contains low numbers of CD27+ memory cells which are the site of EBV persistence in healthy immunocompetent donors. To investigate this apparent paradox, we prospectively monitored EBV levels and B-cell reconstitution in a cohort of allo-HSCT patients for up to 12 months posttransplant. In patients with low or undetectable levels of EBV, the circulating B-cell pool consisted predominantly of transitional and naive cells, with a marked deficiency of CD27+ memory cells which lasted >12 months. However, among patients with high EBV loads, there was a significant increase in both the proportion and number of CD27+ memory B cells. Analysis of sorted CD27+ memory B cells from these patients revealed that this population was preferentially infected with EBV, expressed EBV latent transcripts associated with B-cell growth transformation, had a plasmablastic phenotype, and frequently expressed the proliferation marker Ki-67. These findings suggest that high-level EBV reactivation following allo-HSCT may drive the expansion of latently infected CD27+ B lymphoblasts in the peripheral blood.
Item Type: | Article |
---|---|
Additional Information: | The full text of the published version is available ‘open archive’ via the publisher's website (under ‘Official URL’). |
Uncontrolled Discrete Keywords: | Free Research Articles, human, Immunobiology, Immunotherapy, Plenary Papers, Transplantation, allopurinol, antigens, cd27, b-lymphocytes, hematopoietic stem cell transplantation, herpesvirus 4, memory, allogeneic hematopoietic stem cell transplant |
Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
Related URLs: | |
Copyright Info: | © 2015 by The American Society of Hematology, The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 USC section 1734. |
Depositing User: | Joanne Whittaker |
Date Deposited: | 13 Mar 2024 12:01 |
Last Modified: | 20 Mar 2024 13:15 |
URI: | https://eprints.worc.ac.uk/id/eprint/13713 |
Actions (login required)
View Item |