University of Worcester Worcester Research and Publications

Endophytic Bacteria Expressing β-glucuronidase Cause False Positives in Transformation of Dioscorea Species

Tör, M. ORCID:, Mantell, S.H. and Ainsworth, C.C. (1992) Endophytic Bacteria Expressing β-glucuronidase Cause False Positives in Transformation of Dioscorea Species. Plant Cell Reports, 11 (9). pp. 452-456. ISSN Print 0721-7714 Online 1432-203X

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False positive transformants obtained during plant transformation experiments on species of the monocotyledonous genus Dioscorea (yam) are described. The false positive results were found to be due to endophytic bacteria which exist within aseptically micropropagated shoot cultures and which express -glucuronidase (GUS). The bacteria were isolated and identified as two species of Curtobacterium. The expression of GUS in these organisms was found to be induced by a variety of glucuronide substrates. The induction of GUS activity in the bacteria can be inhibited by chloramphenicol, tetracycline, ticarcillin and sodium azide. Implications of these results for use of the gus gene in plant transformation work are discussed.

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Originally deposited as joint division with National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Discrete Keywords: Glucuronidase, Dioscorea, endophytic bacteria, false positive transformants
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
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Depositing User: Janet Davidson
Date Deposited: 19 Jan 2011 20:07
Last Modified: 17 Jun 2020 16:54

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