University of Worcester Worcester Research and Publications

Two TIR:NB:LRR Genes are Required to Specify Resistance to Peronospora Parasitica Isolate Cala2 in Arabidopsis

Sinapidou, E., Williams, K., Nott, L., Bahkt, S., Tör, M. ORCID:, Crute, P., Bittner-Eddy, P. and Beynon, J. (2004) Two TIR:NB:LRR Genes are Required to Specify Resistance to Peronospora Parasitica Isolate Cala2 in Arabidopsis. The Plant Journal, 38 (6). pp. 898-909. ISSN 0960-7412

Full text not available from this repository. (Request a copy)


Resistance responses that plants deploy in defence against pathogens are often triggered following a recognition event mediated by resistance (R) genes. The encoded R proteins usually contain a nucleotide-binding site (NB) and a leucine-rich repeat (LRR) domain. They are further classified into those that contain an N-terminal coiled coil (CC) motif or a Toll interleukin receptor (TIR) domain. Such R genes, when transferred into a susceptible plant of the same or closely related species, usually impart full resistance capability. We have used map-based cloning and mutation analysis to study the recognition of Peronospora parasitica (RPP)2 (At) locus in Arabidopsis accession Columbia (Col-0), which is a determinant of specific recognition of P. parasitica (At) isolate Cala2. Genetic mapping located RPP2 to a 200-kb interval on chromosome 4, which contained four adjacent TIR:NB:LRR genes. Mutational analysis revealed three classes of genes involved in specifying resistance to Cala2. One class, which resulted in pleiotropic effects on resistance to other P. parasitica (At) isolates, was unlinked to the RPP2 locus; this class included AtSGT1b. The other two classes were mapped within the interval and were specific to Cala2 resistance. Representatives of each of these classes were sequenced, and mutations were found in one or the other of two (RPP2A and RPP2B) of the four TIR:NB:LRR genes. RPP2A and RPP2B complemented their specific mutations, but failed to impart resistance when present alone, and it is concluded that both genes are essential determinants for isolate-specific recognition of Cala2. RPP2A has an unusual structure with a short LRR domain at the C-terminus, preceded by two potential but incomplete TIR:NB domains. In addition, the RPP2A LRR domain lacks conserved motifs found in all but three other TIR:NB:LRR class proteins. In contrast, RPP2B has a complete TIR:NB:LRR structure. It is concluded that RPP2A and RPP2B cooperate to specify Cala2 resistance by providing recognition or signalling functions lacked by either partner protein.

Item Type: Article
Additional Information:

This is an Open Access article. The full-text can be accessed via the Official URL.

Originally deposited as a joint division with National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Discrete Keywords: Arabidopsis, digenic resistance, R genes, downy mildew, NB:LRR, Peronospora.
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
Depositing User: Janet Davidson
Date Deposited: 19 Jan 2011 19:50
Last Modified: 17 Jun 2020 16:54

Actions (login required)

View Item View Item
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.