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An RNAi suppressor activates in planta virus–mediated gene editing

Zhang, X., Kang, L., Zhang, Q., Meng, Q., Pan, Y., Yu, Z., Shi, N., Jackson, S., Zhang, X., Wang, H., Tör, M. and Hong, Yiguo (2019) An RNAi suppressor activates in planta virus–mediated gene editing. Functional & Integrative Genomics. ISSN Print: 1438-793X Online: 1438-7948 (In Press)

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Abstract

RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.

Item Type: Article
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Uncontrolled Keywords: CRISPR/Cas9, FoMV, VmGE, RNAi suppressor
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
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Depositing User: Mahmut Tor
Date Deposited: 19 Dec 2019 11:41
Last Modified: 10 Jan 2020 11:36
URI: https://eprints.worc.ac.uk/id/eprint/9008

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