University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Geminiviral V2 Protein Suppresses Transcriptional Gene Silencing through Interaction with AGO4

Wang, Y., Wu, Y., Gong, Q., Ismayil, A., Yuan, Y., Lian, B., Jia, Q., Han, M., Deng, H., Hong, Yiguo, Hanley-Bowdoin, L., Qi, Y. and Liu, Y. (2019) Geminiviral V2 Protein Suppresses Transcriptional Gene Silencing through Interaction with AGO4. Journal of Virology, 93 (6). e01675-18. ISSN Print: 0022-538X Online: 1098-5514

Full text not available from this repository. (Request a copy)

Abstract

In plants, RNA-directed DNA methylation (RdDM)-mediated transcriptional gene silencing (TGS) is a natural antiviral defense against geminiviruses. Several geminiviral proteins have been shown to target the enzymes related to the methyl cycle or histone modification; however, it remains largely unknown whether and by which mechanism geminiviruses directly inhibit RdDM-mediated TGS. In this study, we showed that Cotton leaf curl Multan virus (CLCuMuV) V2 directly interacts with Nicotiana benthamiana AGO4 (NbAGO4) and that the L76S mutation in V2 (V2L76S) abolishes such interaction. We further showed that V2, but not V2L76S, can suppresses RdDM and TGS. Silencing of NbAGO4 inhibits TGS, reduces the viral methylation level, and enhances CLCuMuV DNA accumulation. In contrast, the V2L76S substitution mutant attenuates CLCuMuV infection and enhances the viral methylation level. These findings reveal that CLCuMuV V2 contributes to viral infection by interaction with NbAGO4 to suppress RdDM-mediated TGS in plants.IMPORTANCE In plants, the RNA-directed DNA methylation (RdDM) pathway is a natural antiviral defense mechanism against geminiviruses. However, how geminiviruses counter RdDM-mediated defense is largely unknown. Our findings reveal that Cotton leaf curl Multan virus V2 contributes to viral infection by interaction with NbAGO4 to suppress RNA-directed DNA methylation-mediated transcriptional gene silencing in plants. Our work provides the first evidence that a geminiviral protein is able to directly target core RdDM components to counter RdDM-mediated TGS antiviral defense in plants, which extends our current understanding of viral counters to host antiviral defense.

Item Type: Article
Additional Information:

The full-text cannot be supplied for this item. Please check availability with your local library or Interlibrary Requests Service.

Uncontrolled Keywords: Argonaute 4, CLCuMuV, RdDM pathway, TGS, V2
Divisions: Academic Departments > Institute of Science and the Environment
Related URLs:
Depositing User: Yiguo Hong
Date Deposited: 26 Aug 2019 08:11
Last Modified: 26 Aug 2019 08:11
URI: https://eprints.worc.ac.uk/id/eprint/8537

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.