Herbert, Rob and Francis, D and Ormrod, J.C. (1992) Cellular and Morphological Changes at the Terminal Shoot Apex of the Short-Day Plant Pharbitis nil During the Transition to Flowering. Physiologia Plantarum, 86 (1). pp. 85-92. ISSN 0031-9317
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Official URL: http://www3.interscience.wiley.com/journal/1193221...
Changes in morphology and measurements of cell doubling time were recorded for the first time in the terminal shoot apex of the short-day plant, Pharbitis nil Chois. (Ipomea nil L.) cv. Violet, undergoing the floral transition. A treatment comprising 48 h darkness given to 4-day-old plants resulted in 100% flowering at the shoot terminal meristem. An inhibitory treatment comprising two 5 min red night-breaks during the 48 h dark period was used to discriminate between events essential for flowering, and those changes resulting from shifts from light to darkness and vice versa. Morphology was studied using both light microscopy and scanning electron microscopy. Cell doubling times were measured using the colchicine accumulation of metaphases method. An increase in the rate of primordial initiation, a change in the divergence angle and a change in phyllotaxis occurred during the floral transition. Moreover, the apex widened and flattened following the inductive dark treatment; the cell doubling time decreased in the peripheral zone and increased in the central zone of these pre-floral meristems.
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|Uncontrolled Keywords:||floral transition, Pharbitis nil, phyllotaxis, red-light, shoot apex, shortday plant|
|Subjects:||Q Science > QK Botany|
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Deposited By:||Rob Herbert|
|Deposited On:||27 Nov 2009 14:36|
|Last Modified:||27 Nov 2009 14:36|
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