Tseng, Hua-Ming and Gattolin, Stefano and Newbury, John and David A., Barrett (2009) Analysis of Mono-, Di- and Oligosaccharides by CE Using a Two-Stage Derivatization Method and LIF Detection. Electrophoresis, 30. pp. 1-7. ISSN 0173-0835 (print), 1522-2683 (online)
A sensitive CE with LIF method has been developed for quantitative analysis of small carbohydrates. In this work, 17 carbohydrates including mono-, di- and oligosaccharides were simultaneously derivatized with 4-fluoro 7-nitrobenzo furazane (NBD-F) via a twostep reaction involving reductive amination with ammonia followed by condensation with NBD-F. Under the optimized derivatization conditions all carbo-hydrates were successfully derivatized within 2.5 h and separated within 15 min using borate buffer (90 mmol/L, pH 9.2). For sugar standards LODs were in the range of 49.7 to 243.6 nmol/L. Migration time and peak area reproducibility were better than RSD 0.1 and 3%, respectively. The method was applied to measure sugars in nanoliter volume samples of phloem sap obtained by stylectomy from wheat and to honeydew samples obtained from aphids feeding from wheat and willow.
Published online on March 24th 2009 by Wiley InterScience in Early View (Articles online in advance of print).
|Uncontrolled Keywords:||carbohydrates, CE, honeydew, LIF, phloem sap|
|Subjects:||T Technology > TP Chemical technology
Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Depositing User:||John Newbury|
|Date Deposited:||02 Apr 2009 10:31|
|Last Modified:||31 Mar 2010 05:00|
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