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Accumulation of Calcium in the Centre of Leaves of Coriander (Coriandrum sativum L.) is Due to an Uncoupling of Water and Ion Transport.

Kerton, Matt and Newbury, John and Hand, David and Pritchard, Jeremy (2009) Accumulation of Calcium in the Centre of Leaves of Coriander (Coriandrum sativum L.) is Due to an Uncoupling of Water and Ion Transport. Journal of Experimental Botany, 60 (1). pp. 227-235. ISSN Online 1460-2431 - Print 0022-0957

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Abstract

The aim of this study is to understand the parameters regulating calcium ion distribution in leaves. Accumulation of ions in leaf tissue is in part dependent on import from the xylem. This import via the transpiration stream is more important for ions such as calcium that are xylem but not phloem mobile and cannot therefore be retranslocated. Accumulation of calcium was measured on bulk coriander leaf tissue (Coriandrum sativum L. cv. Lemon) using ion chromatography and calcium uptake was visualized using phosphorimages of 45Ca2+. Leaves of plants grown in hydroponics had elevated calcium in the centre of the leaf compared with the leaf margin, while K+ was distributed homogeneously over the leaf. This calcium was shown to be localised to the mesophyll vacuoles using EDAX. Stomatal density and evapotranspiration (water loss per unit area of leaf) were equal at inner and outer sections of the leaf. Unequal ion distribution but uniformity of water loss suggested that there was a difference in the extent of uncoupling of calcium and water transport between the inner and outer leaf. Since isolated tissue from the inner and outer leaf were able to accumulate similar amounts of calcium, it is proposed that the spatial variation of leaf calcium concentration is due to differential ion delivery to the two regions rather than tissue/cell-specific differences in ion uptake capacity. There was a positive correlation between whole leaf calcium concentration and the difference in calcium concentration between inner and outer leaf tissue. Exposing the plants to increased humidity reduced transpiration and calcium delivery to the leaf and abolished this spatial variation of calcium concentration. Mechanisms of calcium delivery to leaves are discussed. An understanding of calcium delivery and distribution within coriander will inform strategies to reduce the incidence of calcium-related syndromes such as tip-burn and provides a robust model for the transport of ions and other substances in the leaf xylem.

Item Type: Article
Additional Information:

JXB Advance Access published online on 13th November 2008.

Uncontrolled Keywords: Calcium, Coriandrum sativum, distribution, ion chromatography, leaves, radioisotope, spatial variation, transpiration, uptake.
Subjects: Q Science > QK Botany
Divisions: Academic Departments > Institute of Science and the Environment
Related URLs:
Depositing User: John Newbury
Date Deposited: 30 Jan 2009 09:41
Last Modified: 31 Mar 2010 05:00
URI: https://eprints.worc.ac.uk/id/eprint/556

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