University of Worcester Worcester Research and Publications

Roles of Dicer-Like Proteins 2 and 4 in Intra- and Intercellular Antiviral Silencing

Qin, C., Li, B., Fan, Y., Zhang, X., Yu, Z., Ryabov, E., Zhao, M., Wang, H., Shi, N., Zhang, P., Jackson, S., Tör, M. ORCID:, Cheng, Q., Liu, Y., Gallusci, P. and Hong, Yiguo (2017) Roles of Dicer-Like Proteins 2 and 4 in Intra- and Intercellular Antiviral Silencing. Plant Physiology, 174 (2). pp. 1067-1081. ISSN Print 0032-0889 Online 1532-2548

Plant Physiol.-2017-Qin-pp.17.00475.pdf - Accepted Version
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RNA silencing is an innate antiviral mechanism conserved in organisms across kingdoms. Such cellular defense involves DICER or DICER-LIKEs (DCLs) that process viral RNAs into small interfering (vsi)RNAs. Plants encode four DCLs which play diverse roles in cell-autonomous virus-induced RNA silencing (known as VIGS) against viral invasion. However, intracellular VIGS can spread between cells, and the genetic basis and involvement of vsiRNAs in non-cell autonomous VIGS remains poorly understood. Here using GFP as a reporter gene together with a suite of DCL RNAi transgenic lines, we show that in addition to well-established activities of DCLs in intracellular VIGS and vsiRNA biogenesis, DCL4 inhibits intercellular VIGS whilst DCL2 is required, likely along with DCL2-processed/dependent vsiRNAs and their precursor RNAs, for efficient VIGS trafficking from epidermal to adjacent cells. DCL4 imposed an epistatic effect on DCL2 to impede cell-to-cell spread of VIGS. Our results demonstrate previously unknown functions for DCL2 and DCL4 which may form a dual defensive frontier for intra- and intercellular silencing to double-protect cells from virus infection in Nicotiana benthamiana.

Item Type: Article
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Uncontrolled Discrete Keywords: DICER-LIKEs, vsiRNAs, non-cell autonomous intercellular VIGS, Nicotiana benthamiana
Subjects: Q Science > Q Science (General)
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
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Copyright Info: Open Access article
Depositing User: Janet Davidson
Date Deposited: 09 May 2017 07:06
Last Modified: 17 Jun 2020 17:17

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