University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Fructose Alters Adiponectin, Haptoglobin and Angiotensinogen Gene Expression in 3T3-L1 Adipocytes

Carvalho, C.R. and Bueno, Allain and Mattos, A.M. and Biz, C. and de Oliveira, C. and Pisani, L.P. and Ribeiro, E.B. and Oller do Nascimento, C.M. and Oyama, L.M. (2010) Fructose Alters Adiponectin, Haptoglobin and Angiotensinogen Gene Expression in 3T3-L1 Adipocytes. Nutrition Research, 30 (9). pp. 644-649. ISSN 0271-5317

[img] Text
Fructose alters adiponectin, haptoglobin and angiotensinogen gene.pdf - Published Version
Restricted to Repository staff only

Download (292kB) | Request a copy

Abstract

Fructose- or sucrose-rich diets can cause insulin resistance and increase the risk of cardiovascular disease. Adipokines are correlated with the development of these diseases in obesity. We hypothesize that fructose and sucrose induce insulin resistance via effects on adipokine gene expression in adipocytes. This study analyzed the effect of fructose or glucose on adiponectin, haptoglobin, and angiotensinogen gene expression in 3T3-L1 adipocytes. Ten days after differentiation, the cells were pretreated with serum- and glucose-free medium. Twenty-four hours later, fructose or glucose (0, 5, 10, or 20 mmol) was added into the medium, and the cells were collected after a further 24 hours. Adiponectin, haptoglobin, and angiotensinogen gene expression were determined. Adiponectin gene expression increased when 10 or 20 mmol glucose was added compared with that observed for the non–hexose-treated cells. A similar effect occurred when 5 mmol fructose was added. Glucose (10 mmol) and fructose (20 mmol) stimulated haptoglobin gene expression in 3T3-L1 adipocytes compared with 0 mmol, with glucose producing a more pronounced effect. Although 20 mmol fructose caused an increase in angiotensinogen gene expression, glucose did not. In conclusion, in this study of 2 hexoses revealed an increase in adiponectin gene expression, suggesting that the effect of a glucose-rich diet on the development of insulin resistance is not related to the effect of these hexoses on adipocyte adiponectin gene expression. However, insulin resistance and cardiovascular disease promoted by fructose-rich diets could be partially related to the effect of fructose on adiponectin and angiotensinogen gene expression.

Item Type: Article
Additional Information:

The full-text cannot be supplied for this item. Please check availability with your local library or Interlibrary Requests Service.

Uncontrolled Keywords: glucose, fructose, adiponectin, haptoglobin, angiotensinogen, 3T3-L1 adipocyte, diet, health, nutrition
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
Divisions: Academic Departments > Institute of Science and the Environment
Related URLs:
Depositing User: Tanya Buchanan
Date Deposited: 09 Aug 2016 12:45
Last Modified: 09 Aug 2016 12:45
URI: https://eprints.worc.ac.uk/id/eprint/4762

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.