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The Collection and Retention of a Range of Common Airborne Spore Types Trapped Directly into Microtiter Wells for Enzyme-linked Immunosorbent Analysis

Wakeham, Alison and Kennedy, Roy and McCartney, A. (2004) The Collection and Retention of a Range of Common Airborne Spore Types Trapped Directly into Microtiter Wells for Enzyme-linked Immunosorbent Analysis. Journal of Aerosol Science, 35 (7). pp. 835-850. ISSN 0021-8502

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Abstract

The collection and retention of a range of fungal spores for a novel spore trap (Microtiter immunospore trap (MTIST)), designed for use with immunoassays, was investigated in wind tunnel experiments. Concentrations of spores of Botrytis cinerea, Cladosporium cladosporioides, Lycopodium clavatum, Erysiphe cruciferarum and Penicillium roqueforti, were measured usingthe MTIST and miniature suction traps (same characteristics as a Burkard 7 day volumetric spore trap). A clear relationship was observed between the spore concentrations measure by the MTIST and those measured by the mini-suction traps. No signi7cant (Fprob¿0:4) effect of windspeed was observed for any spore type in samples collected in the MTIST trap. There was some effect on windspeed in the collection of the larger spore types (Lycopodium) as measured by the mini-suction traps. Pre-coating microtiter wells of the MTIST spore trap with a mixture of paraffin wax and petroleum jelly significantly increased the collection of the two largest spore types. There were significant differences between the MTIST and the mini-suction spore traps in their ability to trap the smaller spore types (Cladosporium and Penicillium). Employinga Botrytis-specific antiserum, a high level of correlation was observed between the numbers of Botrytis conidia trapped by the MTIST spore trap and, corresponding absorbance values of an ELISA when, wells were pre-coated with either distilled water, poly-L-lysine or a petroleum jelly and paraffin wax mixture.

Item Type: Article
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Staff and students at the University of Worcester can access the full-text via Library Search. The full-text of this article is available on Level 4 at The Hive in: Wakeham, Alison. The development and application of immunological tests within horticultural crop disease management systems. University of Worcester, PhD by Portfolio, 2014.

External users should check availability with their local library or Interlibrary Requests Service.

Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Keywords: fungal spores, air samplers, spore traps, collection, retention
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
S Agriculture > S Agriculture (General)
Divisions: Academic Departments > Institute of Science and the Environment
Related URLs:
Depositing User: Alison Wakeham
Date Deposited: 19 May 2015 17:33
Last Modified: 15 Feb 2016 14:57
URI: https://eprints.worc.ac.uk/id/eprint/3704

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