University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Variation of the Group 5 Grass Pollen Allergen Content of Airborne Pollen in Relation to Geographical Location and Time in Season

Buters, J.T.M., Prank, M., Sofiev, M., Pusch, G., Albertini, R., Annesi-Maesano, I., Antunes, C.M., Behrendt, H., Berger, U., Brandao, R., Celenk, S., Galán, C., Grewling, Ł., Jackowiak, B., Kennedy, Roy, Rantio-Lehtimäki, A., Reese, G., Sauliene, I., Smith, Matt, Thibaudon, M., Weber, B. and Cecchi, L. (2015) Variation of the Group 5 Grass Pollen Allergen Content of Airborne Pollen in Relation to Geographical Location and Time in Season. Journal of Allergy and Clinical Immunology, 136 (1). pp. 87-95. ISSN 0091-6749

[img] Text
Variation of the group 5 grass polen allergen.pdf
Restricted to Repository staff only

Download (3MB) | Request a copy
[img] Text
Buters 2015.pdf - Published Version
Restricted to Repository staff only

Download (3MB) | Request a copy

Abstract

Allergies to grass pollen are the number one cause of outdoor hay fever. The human immune system reacts with symptoms to allergens from pollen. Objective: We investigated the natural variability in release of the major group 5 allergen from grass pollen across Europe. Methods: Airborne pollen and allergens were simultaneously collected daily with a volumetric spore trap and a high-volume cascade impactor at 10 sites across Europe for 3 consecutive years. Group 5 allergen was determined with a Phl p 5 specific ELISA in two fractions of ambient air: Particulate Matter (PM) >10μm and 10μm>PM>2.5μm. Mediator release by ambient air was determined in FcεR1-humanized basophils. Origin of pollen was modeled and condensed to pollen potency maps. Results: On average grass pollen released 2.3 pg Phl p 5/pollen. Allergen release per pollen (potency) varied substantially, ranging from 0 to 9 pg Phl p 5/pollen (5 to 95% percentile). The main variation was locally day-to-day. Average potency maps across Europe varied between years. Mediator release from basophilic granulocytes correlated better with allergen/m3 (r2=0.80, p<0.001) than with pollen/m3 (r2=0.61, p<0.001). In addition, pollen released different amounts of allergen in the nonpollen bearing fraction of ambient air depending on humidity. Conclusion: Across Europe, the same amount of pollen released substantially different amounts of group 5 grass pollen allergen. This variation in allergen release is on top of variations in pollen counts. Molecular aerobiology, i.e. determining allergen in ambient air, may be a valuable addition to pollen counting.

Item Type: Article
Additional Information:

Staff and students at the University of Worcester can access the full-text via Library Search. External users should check availability with their local library or Interlibrry Requests Service.

Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Keywords: allergen, grass, Phl p 5, Europe, PM10, PM2.5, exposure, allergy, basophil, modeling, SILAM, HIALINE
Subjects: Q Science > Q Science (General)
Divisions: Academic Departments > Institute of Science and the Environment
Related URLs:
Depositing User: Sally Wall
Date Deposited: 09 Apr 2015 11:02
Last Modified: 06 Jan 2017 16:54
URI: https://eprints.worc.ac.uk/id/eprint/3592

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.