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Variation of the Group 5 Grass Pollen Allergen Content of Airborne Pollen in Relation to Geographical Location and Time in Season

Buters, J.T.M. and Prank, M. and Sofiev, M. and Pusch, G. and Albertini, R. and Annesi-Maesano, I. and Antunes, C.M. and Behrendt, H. and Berger, U. and Brandao, R. and Celenk, S. and Galán, C. and Grewling, Ł. and Jackowiak, B. and Kennedy, Roy and Rantio-Lehtimäki, A. and Reese, G. and Sauliene, I. and Smith, Matt and Thibaudon, M. and Weber, B. and Cecchi, L. (2015) Variation of the Group 5 Grass Pollen Allergen Content of Airborne Pollen in Relation to Geographical Location and Time in Season. Journal of Allergy and Clinical Immunology, 136 (1). pp. 87-95. ISSN 0091-6749

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Abstract

Allergies to grass pollen are the number one cause of outdoor hay fever. The human immune system reacts with symptoms to allergens from pollen. Objective: We investigated the natural variability in release of the major group 5 allergen from grass pollen across Europe. Methods: Airborne pollen and allergens were simultaneously collected daily with a volumetric spore trap and a high-volume cascade impactor at 10 sites across Europe for 3 consecutive years. Group 5 allergen was determined with a Phl p 5 specific ELISA in two fractions of ambient air: Particulate Matter (PM) >10μm and 10μm>PM>2.5μm. Mediator release by ambient air was determined in FcεR1-humanized basophils. Origin of pollen was modeled and condensed to pollen potency maps. Results: On average grass pollen released 2.3 pg Phl p 5/pollen. Allergen release per pollen (potency) varied substantially, ranging from 0 to 9 pg Phl p 5/pollen (5 to 95% percentile). The main variation was locally day-to-day. Average potency maps across Europe varied between years. Mediator release from basophilic granulocytes correlated better with allergen/m3 (r2=0.80, p<0.001) than with pollen/m3 (r2=0.61, p<0.001). In addition, pollen released different amounts of allergen in the nonpollen bearing fraction of ambient air depending on humidity. Conclusion: Across Europe, the same amount of pollen released substantially different amounts of group 5 grass pollen allergen. This variation in allergen release is on top of variations in pollen counts. Molecular aerobiology, i.e. determining allergen in ambient air, may be a valuable addition to pollen counting.

Item Type: Article
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Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Keywords: allergen, grass, Phl p 5, Europe, PM10, PM2.5, exposure, allergy, basophil, modeling, SILAM, HIALINE
Subjects: Q Science > Q Science (General)
Divisions: Academic Departments > Institute of Science and the Environment
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Depositing User: Sally Wall
Date Deposited: 09 Apr 2015 11:02
Last Modified: 06 Jan 2017 16:54
URI: https://eprints.worc.ac.uk/id/eprint/3592

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