University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Oligosaccharins and Pectimorf® Stimulate Root Elongation and Shorten the Cell Cycle in Higher Plants

Herbert, Rob (2012) Oligosaccharins and Pectimorf® Stimulate Root Elongation and Shorten the Cell Cycle in Higher Plants. Plant Growth Regulation, 68 (2). pp. 211-221. ISSN 0167-6903 (print version) ISSN: 1573-5087 (electronic version)

Full text not available from this repository. (Request a copy)

Abstract

The aim was to test promotive effects of oligosaccharins on root growth and development at the root apical meristem and the cell cycle using the model systems, Arabidopsis thaliana and the tobacco (Nicotiana tabacum) BY-2 cell line. Arabidopsis was grown on medium supplemented with 0.1 mg L−1 oligoxyloglucan (OX), 10 mg L−1 Pectimorf® (P) or 0.5 mg L−1 indole butyric acid (IBA). Primary root length, number of lateral root primordia, root apical meristem (RAM) length and epidermal cell length were recorded. Three genotypes were used: wild type (WT) and transgenic lines expressing either Schizosaccharomyces pombe (Sp) cdc25 or over-expressing(oe) Arath;WEE1. All treatments promoted primary root elongation but repressed lateral root production. Only P had a clear positive effect on meristem length whereas all other genotype × treatment interactions showed shorter RAMs. Whilst IBA, OX and P induced an increase in cell length in Spcdc25, the same treatments caused a significant decrease in WEE1 oe . Mitotic indices were also significantly higher in roots treated with oligosaccharins suggesting a shortening of the cell cycle. This hypothesis was tested in the BY-2 cell line. Both OX and P shortened the cell cycle exclusively through a shortening of G1 whilst mitotic cell size remained constant between treatments. In conclusion, both OX and P do indeed stimulate growth and shorten the cell cycle in higher plants and at the cellular level are able to reverse large and small cell size phenotypes normally exhibited by WEE1 oe and Spcdc25 genotypes, respectively.

Item Type: Article
Additional Information:

The full-text cannot be supplied for this item. Please check availability with your local library or Interlibrary Requests Service.

Uncontrolled Keywords: Arabidopsis thaliana, BY-2 cells, Cell cycle, Lateral roots, Nicotiana, tabacum, Oligosaccharins, Plant growth, regulators, Root elongation, Root morphology
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Academic Departments > Institute of Science and the Environment
Depositing User: Rob Herbert
Date Deposited: 25 Jun 2013 09:09
Last Modified: 12 Jul 2013 05:01
URI: https://eprints.worc.ac.uk/id/eprint/2293

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.