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Gene Dosage Effect of WEE1 on Growth and Morphogenesis from Arabidopsis Hypocotyl Explants.

Spadafora, Natasha and Perrota , Lara and Nieuwland, Jeroen and Albini, Diego and Bitonti , Beatrice M. and Herbert, Rob and Doonan, John and Marchbank, Angela and Siciliano , Ilario and Lentz Gronlund, Anne and Francis, Dennis and Rogers, Hilary (2012) Gene Dosage Effect of WEE1 on Growth and Morphogenesis from Arabidopsis Hypocotyl Explants. Annals of Botany, 110 (8). pp. 1631-1639. ISSN Print: 0305-7364 Online: 1095-8290

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Abstract

Background and Aims How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain- and loss-of-function WEE1 arabidopsis lines both in vivo and in vitro. Methods Hypocotyl explants from an over-expressing Arath;WEE1 line (WEE1oe), two T-DNA insertion lines (wee1-1 and wee1-4) and wild type (WT) were cultured on two-way combinations of kinetin and naphthyl acetic acid. Root growth and meristematic cell size were also examined. Key Results Quantitative data indicated a repressive effect in WEE1oe and a significant increase in morphogenetic capacity in the two T-DNA insertion lines compared with WT. Compared with WT, WEE1oe seedlings exhibited a slower cell-doubling time in the root apical meristem and a shortened primary root, with fewer laterals, whereas there were no consistent differences in the insertion lines compared with WT. However, significantly fewer adventitious roots were recorded for WEE1oe and significantly more for the insertion mutant wee1-1. Compared with WT there was a significant increase in meristem cell size in WEE1oe for all three ground tissues but for wee1-1 only cortical cell size was reduced. Conclusions There is a gene dosage effect of WEE1 on morphogenesis from hypocotyls both in vitro and in vivo.

Item Type: Article
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First published online October 11, 2012

The full-text can be accessed via the official URL.

Uncontrolled Keywords: Arabidopsis thaliana, cell cycle, development, growth, hypocotyl, tissue culture, WEE1.
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Departments > Institute of Science and the Environment
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Copyright Info: Published by Oxford University Press on behalf of the Annals of Botany Company
Depositing User: Rob Herbert
Date Deposited: 29 Oct 2012 17:58
Last Modified: 13 Nov 2014 14:53
URI: https://eprints.worc.ac.uk/id/eprint/2008

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