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Development of Detection Systems for the Sporangia of Peronospora Destructor

Kennedy, Roy and Wakeham, Alison (2008) Development of Detection Systems for the Sporangia of Peronospora Destructor. European Journal of Plant Pathology, 122 (1). pp. 147-155. ISSN Print: 0929-1873 Online:1573-8469

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Abstract

A monoclonal antibody that recognises components of the wall of sporangia of Peronospora destructor was raised. Tests using spores of higher fungi and other species of mildew demonstrated the specificity of the monoclonal. The antibody was used to develop lateral flow devices for sporangia of P. destructor. A competitive lateral flow format was developed which could detect onion downy mildew sporangia. Five-microliter gold anti-mouse IgM solution pre-mixed with 10 μl of P. destructor monoclonal antibody (EMA 242) proved the optimal concentration for detection of sporangia of P. destructor when applied to sample pads of lateral flow devices. Limits of approximately 500 sporangia of P. destructor could be detected by the absence of a test line on the lateral flow device within test samples. Using a scanning densitometer improved the sensitivity of detection. Further development and validation of the test is required if it is to be used for risk assessments of onion downy mildew in the field.

Item Type: Article
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Uncontrolled Keywords: onion downy mildew, monoclonal antibody, Peronospora destructor, lateral flow assay, detection, PTA ELISA, immunofluorescence
Subjects: S Agriculture > S Agriculture (General)
Divisions: Research Centres > National Pollen and Aerobiology Research Unit
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Depositing User: Sally Wall
Date Deposited: 15 Mar 2012 11:41
Last Modified: 15 Mar 2012 11:51
URI: https://eprints.worc.ac.uk/id/eprint/1586

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