University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Identification of the Pollen Self-incompatibility Determinant in Papaver rhoeas.

Wheeler, Mike and de Graaf, Barend and Hadjiosif, Natalie and Perry , Ruth and Poulter, Natalie and Osman, Kim and Vatovec, Sabina and Harper, Andrea and Franklin, Christopher and Franklin-Tong, Veronica (2009) Identification of the Pollen Self-incompatibility Determinant in Papaver rhoeas. Nature, 459. pp. 992-995. ISSN Print: 0028-0836 Online: 1476-4687

Full text not available from this repository. (Request a copy)

Abstract

Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus1,2. ‘Self’ (incompatible) pollen is discriminated from ‘non-self’ (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequentlyinhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca21-dependent signalling network, resulting in pollen inhibition and programmed cell death3–7. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel 20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell–cell recognition determinant it contributes to the available information concerning the origins and evolution of cell–cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.

Item Type: Article
Additional Information:

The electronic full-text cannot be supplied for this item. Please check availability with your local library or Interlibrary Requests Service.

Uncontrolled Keywords: self-incompatibility, cell-cell recognition, Papaver rhoeas, genetics
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Academic Departments > Institute of Science and the Environment
Depositing User: Mike Wheeler
Date Deposited: 01 Nov 2011 08:10
Last Modified: 01 Nov 2011 08:10
URI: https://eprints.worc.ac.uk/id/eprint/1488

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.