Wheeler, Mike and de Graaf, Barend and Hadjiosif, Natalie and Perry , Ruth and Poulter, Natalie and Osman, Kim and Vatovec, Sabina and Harper, Andrea and Franklin, Christopher and Franklin-Tong, Veronica (2009) Identification of the Pollen Self-incompatibility Determinant in Papaver rhoeas. Nature, 459 . pp. 992-995. ISSN Print: 0028-0836 Online: 1476-4687
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Official URL: http://www.nature.com/nature/journal/v459/n7249/fu...
Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus1,2. ‘Self’ (incompatible) pollen is discriminated from ‘non-self’ (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequentlyinhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca21-dependent signalling network, resulting in pollen inhibition and programmed cell death3–7. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel 20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell–cell recognition determinant it contributes to the available information concerning the origins and evolution of cell–cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.
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|Uncontrolled Keywords:||self-incompatibility, cell-cell recognition, Papaver rhoeas, genetics|
|Subjects:||Q Science > QH Natural history > QH426 Genetics|
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Deposited By:||Mike Wheeler|
|Deposited On:||01 Nov 2011 08:10|
|Last Modified:||01 Nov 2011 08:10|
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