Evans, K., Ransom, E., Nayakoti, S., Wilding, B., Mohd Salleh, F., Gržina, I., Erber, L., Tse, C., Hill, C., Polanski, K., Holland, A., Bukhat, S., Herbert, Rob, de Graaf, B., Denby, K., Buchanan-Wollaston, V. and Rogers, H. (2024) Expression of the Arabidopsis redox-related LEA protein, SAG21 is regulated by ERF, NAC and WRKY transcription factors. Scientific Reports, 14 (7756). ISSN 2045-2322
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Abstract
SAG21/LEA5 is an unusual late embryogenesis abundant protein in Arabidopsis thaliana, that is primarily mitochondrially located and may be important in regulating translation in both chloroplasts and mitochondria. SAG21 expression is regulated by a plethora of abiotic and biotic stresses and plant growth regulators indicating a complex regulatory network. To identify key transcription factors regulating SAG21 expression, yeast-1-hybrid screens were used to identify transcription factors that bind the 1685 bp upstream of the SAG21 translational start site. Thirty-three transcription factors from nine different families bound to the SAG21 promoter, including members of the ERF, WRKY and NAC families. Key binding sites for both NAC and WRKY transcription factors were tested through site directed mutagenesis indicating the presence of cryptic binding sites for both these transcription factor families. Co-expression in protoplasts confirmed the activation of SAG21 by WRKY63/ABO3, and SAG21 upregulation elicited by oligogalacturonide elicitors was partially dependent on WRKY63, indicating its role in SAG21 pathogen responses. SAG21 upregulation by ethylene was abolished in the erf1 mutant, while wound-induced SAG21 expression was abolished in anac71 mutants, indicating SAG21 expression can be regulated by several distinct transcription factors depending on the stress condition.
Item Type: | Article |
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Uncontrolled Discrete Keywords: | Abiotic stress, Biotic stress, Ethylene, NAC transcription factors, SAG21/LEA5, WRKY transcription factors |
Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
Related URLs: | |
Copyright Info: | Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License |
Depositing User: | Rob Herbert |
Date Deposited: | 10 Apr 2024 14:24 |
Last Modified: | 11 Apr 2024 08:24 |
URI: | https://eprints.worc.ac.uk/id/eprint/13824 |
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