University of Worcester Worcester Research and Publications

Defending the Arabidosolanaceae (Poster presentation)

Jones, J.D.G., Rivas, S., Hesse-Peck, A., Rowland, O., Rujon, A., Ludwig, A., Sadanandon, A., Gonzales-Lamother, R., van der Berg, H., Golstein, C., Navarro, L., Sjölander, K., Tör, M. ORCID: and Holub, E. (2004) Defending the Arabidosolanaceae (Poster presentation). In: Plant & Animal Genomes XII Conference, 10th-14th January 2004, San Diego, California. (Unpublished)

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Tomato Cf-genes confer race-specific resistance to Cladosporium fulvum. Resistance is mediated through recognition of specific products of fungal avirulence (Avr) genes. Cf-genes encode plasma membrane receptor-like proteins (RLPs), with an LRR domain that is involved in recognition, and no apparent signalling domain. These proteins are difficult to investigate purely by biochemical approaches. To understand any biological mechanism, it is crucial to choose the right biological system. Our studies on Cf-genes have revealed the existence of an RLP-based mechanism, the full understanding of which now requires deployment of a better system for forward and reverse genetics, namely Arabidopsis. The Arabidopsis Col-0 set of 57 RLPs has been defined and subjected to computational analysis. From analysis of genetic variation for Peronospora resistance in Arabidopsis, the RPP27 gene was defined (in Landsberg), and it also encodes an RLP. This observation opens up the possibility of forward genetics approaches to reveal RLP mechanisms. Reverse genetics approaches are also being undertaken. In Cf-9-carrying tobacco cell cultures, provision of Avr9 results in activation of a set of defence responses, including production of active oxygen, gene induction and cell death within 3–4 hrs. This cell death is prevented by proteasome inhibitors. Expression profiling of elicited tobacco cells revealed a set of Avr9, Cf-9 Rapidly Elicited (ACRE) genes. Virus-induced gene silencing (VIGS) was used to show that several E3 ubiquitin ligase genes, and a protein kinase, are required for cell death. In Arabidopsis cell cultures challenged with flagellin or a conserved flagellin peptide (flg22), similar defense responses are elicited. Arabidopsis orthologs of ACRE (AtACRE) genes are being investigated to assess their role in RPP27-dependent and other disease resistance.

Item Type: Conference or Workshop Item (Poster)
Additional Information:

Originally deposited as a joint division with National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Discrete Keywords: genetic variation, plant genomes, Arabidopsis
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
Depositing User: Janet Davidson
Date Deposited: 03 Mar 2011 12:29
Last Modified: 17 Jun 2020 16:54

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