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Gene Transfer Into Citrus (Citrus limon L.) Nucellar Cells by Particle Bombardment and Expression of Gus Activity

Kayim, M and Koc, N.K. and Tör, M (1996) Gene Transfer Into Citrus (Citrus limon L.) Nucellar Cells by Particle Bombardment and Expression of Gus Activity. Turkish Journal of Agriculture and Forestry, 20. pp. 349-352. ISSN Print: 1300-011X Online: 1303-6173

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Abstract

The presence of foreign genes that were transferred into Citrus nucellar cells by the particle bombardment system has been expressed with the GUS assay. Tungsten microcarriers coated with plasmid DNA containing the gus A gene (pBI221.2) were accelerated at high velocity using a biolistic device into lemon (Citrus limon var. Kütdiken) target cells. Histochemical examination of the bombarded nucellar cells revealed that only several cell aggregates had cells expressing the gus A gene. Control cell aggregates that were not bombarded did not exhibit any cells expressing the gus A gene.

Item Type: Article
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Uncontrolled Keywords: gene transfer, Citrus nucellar cells, GUS activity
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Divisions: Academic Departments > Institute of Science and the Environment
Research Centres > National Pollen and Aerobiology Research Unit
Depositing User: Janet Davidson
Date Deposited: 19 Jan 2011 20:03
Last Modified: 19 Jan 2011 20:03
URI: https://eprints.worc.ac.uk/id/eprint/1177

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