Kayim, M and Koc, N.K. and Tör, M (1996) Gene Transfer Into Citrus (Citrus limon L.) Nucellar Cells by Particle Bombardment and Expression of Gus Activity. Turkish Journal of Agriculture and Forestry, 20. pp. 349-352. ISSN Print: 1300-011X Online: 1303-6173Full text not available from this repository. (Request a copy)
The presence of foreign genes that were transferred into Citrus nucellar cells by the particle bombardment system has been expressed with the GUS assay. Tungsten microcarriers coated with plasmid DNA containing the gus A gene (pBI221.2) were accelerated at high velocity using a biolistic device into lemon (Citrus limon var. Kütdiken) target cells. Histochemical examination of the bombarded nucellar cells revealed that only several cell aggregates had cells expressing the gus A gene. Control cell aggregates that were not bombarded did not exhibit any cells expressing the gus A gene.
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|Uncontrolled Keywords:||gene transfer, Citrus nucellar cells, GUS activity|
|Subjects:||Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
|Divisions:||Academic Departments > Institute of Science and the Environment
Research Centres > National Pollen and Aerobiology Research Unit
|Depositing User:||Janet Davidson|
|Date Deposited:||19 Jan 2011 20:03|
|Last Modified:||19 Jan 2011 20:03|
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