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Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy mildew) Isolates

Bittner-Eddy, P and Can, C and Gunn, N and Pinel, M and Tör, M and Crute, I and Holub, E.B. and Beynon, J (1999) Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy mildew) Isolates. Molecular Plant-Microbe Interactions, 12 (9). pp. 792-802. ISSN 0894-0282

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Abstract

Fifteen isolates of the biotrophic oomycete Peronospora parasitica (downy mildew) were obtained from a population of Arabidopsis thaliana plants that established naturally in a garden the previous year. They exhibited phenotypic variation in a set of 12 Arabidopsis accessions that suggested that the parasite population consisted of at least six pathotypes. One isolate, Maks9, elicited an interaction phenotype of flecking necrosis and no sporulation (FN) in the Arabidopsis accession Nd-1, and more extensive pitting necrosis with no sporulation (PN) in the accession Ws-2. RPP13 was designated as the locus for a single dominant resistance gene associated with the resistance in Nd-1 and mapped to an interval of approximately 60 kb on a bacterial artificial chromosome (BAC) contig on the lower arm of chromosome 3. This locus is approximately 6 cM telomeric to RPP1, which was previously described as the locus for the PN interaction with five Peronospora isolates, including resistance to Maks9 in Ws-2. New Peronospora isolates were obtained from four other geographically distinct populations of P. parasitica. Four isolates were characterized that elicited an FN phenotype in Nd-1 and mapped resistance to the RPP13 locus. This suggests that the RPP13 locus contains either a single gene capable of multiple isolate recognition or a group of tightly linked genes. Further analysis suggests that the RPP11 gene in the accession Rld-0 may be allelic to RPP13 but results in a different recognition capability.

Item Type: Article
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This is an Open Access article. The full-text is available via the Official URL.

Uncontrolled Keywords: genetic mapping, physical mapping, RPP13 locus, Arabidopsis, plant-pathogen relationships
Subjects: Q Science > QK Botany
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Divisions: Academic Departments > Institute of Science and the Environment
Research Centres > National Pollen and Aerobiology Research Unit
Depositing User: Janet Davidson
Date Deposited: 19 Jan 2011 20:00
Last Modified: 20 Jan 2011 11:26
URI: https://eprints.worc.ac.uk/id/eprint/1174

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