Gronier, B., Savignac, H.M., Di Miceli, Mathieu ORCID: https://orcid.org/0000-0003-3713-0370, Idriss, S.M., Tzortzis, G., Anthony, D. and Burnet, P.W.J.
(2018)
Increased cortical neuronal responses to NMDA and improved attentional set-shifting performance in rats following prebiotic (B-GOS) ingestion.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 28 (1).
pp. 211-224.
ISSN 0924-977X
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Gronier 2017 - Increased cortical neuronal responses to NMDA and improved attentional set-shifting performance in rats following prebiotic B-GOS ingestion.pdf Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
Abstract
We have previously shown that prebiotics (dietary fibres that augment the growth of indigenous beneficial gut bacteria) such as Bimuno galacto-oligosaccharides (B-GOS), increased N-methyl-D-aspartate (NMDA) receptor levels in the rat brain. The current investigation examined the functional correlates of these changes in B-GOS-fed rats by measuring cortical neuronal responses to NMDA using in vivo NMDA micro-iontophoresis electrophysiology, and performance in the attentional set-shifting task. Adult male rats were supplemented with B-GOS in the drinking water 3 weeks prior to in vivo iontophoresis or behavioural testing. Cortical neuronal responses to NMDA iontophoresis, were greater (+30%) in B-GOS administered rats compared to non-supplemented controls. The intake of B-GOS also partially hindered the reduction of NMDA responses by the glycine site antagonist, HA-966. In the attentional set-shifting task, B-GOS -fed rats shifted from an intra-dimensional to an extra-dimensional set in fewer trials than controls, thereby indicating greater cognitive flexibility. An initial exploration into the mechanisms revealed that rats ingesting B-GOS had increased levels of plasma acetate, and cortical GluN2B subunits and Acetyl Co-A Carboxylase mRNA. These changes were also observed in rats fed daily for 3 weeks with glyceryl triacetate, though unlike B-GOS, cortical histone deacetylase (HDAC1, HDAC2) mRNAs were also increased which suggested an additional epigenetic action of direct acetate supplementation. Our data demonstrate that a pro-cognitive effect of B-GOS intake in rats is associated with an increase in cortical NMDA receptor function, but the role of circulating acetate derived from gut bacterial fermentation of this prebiotic requires further investigation.
Item Type: | Article |
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Additional Information: | The full-text of the published version of the article can be accessed via the Official URL. |
Uncontrolled Discrete Keywords: | electrophysiology, cognition, short-chain fatty acid, microbiota, glutamate |
Divisions: | College of Health, Life and Environmental Sciences > School of Science and the Environment |
Related URLs: | |
Copyright Info: | Open Access article |
Depositing User: | Mathieu Di Miceli |
Date Deposited: | 06 Oct 2021 11:17 |
Last Modified: | 07 Dec 2021 16:53 |
URI: | https://eprints.worc.ac.uk/id/eprint/11400 |
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