University of Worcester Worcester Research and Publications

Small RNA Inhibits Infection by Downy Mildew Pathogen Hyaloperonospora Arabidopsidis

Bilir, Ozlem, Telli, Osman, Norman, Chris, Budak, H., Hong, Yiguo and Tör, M. ORCID: (2019) Small RNA Inhibits Infection by Downy Mildew Pathogen Hyaloperonospora Arabidopsidis. Molecular Plant Pathology, 20 (11). pp. 1523-1534. ISSN Online: 1364-3703

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Gene silencing exists in eukaryotic organisms as a conserved regulation of the gene expression mechanism. In general, small RNAs (sRNAs) are produced within the eukaryotic cells and incorporated into an RNA‐induced silencing complex (RISC) within cells. However, exogenous sRNAs, once delivered into cells, can also silence target genes via the same RISC. Here, we explored this concept by targeting the Cellulose synthase A3 (CesA3) gene of Hyaloperonospora arabidopsidis (Hpa), the downy mildew pathogen of Arabidopsis thaliana. Hpa spore suspensions were mixed with sense or antisense sRNAs and inoculated onto susceptible Arabidopsis seedlings. While sense sRNAs had no obvious effect on Hpa pathogenicity, antisense sRNAs inhibited spore germination and hence infection. Such inhibition of infection was not race‐specific, but dependent on the length and capping of sRNAs. Inhibition of infection by double stranded sRNA was more efficient than that observed with antisense sRNA. Thus, exogenous sRNA targeting conserved CesA3 could suppress Hpa infection in Arabidopsis, indicating the potential of this simple and efficient sRNA‐based approach for deciphering gene functions in obligate biotrophic pathogens as well as for R‐gene independent control of diseases in plants.

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© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Uncontrolled Discrete Keywords: Arabidopsis, downy mildew, oomycetes, spray-induced gene silencing (SIGA), sRNA, SERG
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
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Copyright Info: Open Access article (UW LS APC)
Depositing User: Mahmut Tor
Date Deposited: 03 Oct 2019 14:25
Last Modified: 25 Oct 2022 10:54

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