University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Small RNA Inhibits Infection by Downy Mildew Pathogen Hyaloperonospora Arabidopsidis

Bilir, Ozlem, Telli, Osman, Norman, Chris, Budak, H., Hong, Yiguo and Tör, M. (2019) Small RNA Inhibits Infection by Downy Mildew Pathogen Hyaloperonospora Arabidopsidis. Molecular Plant Pathology, 20 (11). pp. 1523-1534. ISSN Online: 1364-3703

[img]
Preview
Text
Bilir_et_al-2019-Molecular_Plant_Pathology.pdf
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Gene silencing exists in eukaryotic organisms as a conserved regulation of the gene expression mechanism. In general, small RNAs (sRNAs) are produced within the eukaryotic cells and incorporated into an RNA‐induced silencing complex (RISC) within cells. However, exogenous sRNAs, once delivered into cells, can also silence target genes via the same RISC. Here, we explored this concept by targeting the Cellulose synthase A3 (CesA3) gene of Hyaloperonospora arabidopsidis (Hpa), the downy mildew pathogen of Arabidopsis thaliana. Hpa spore suspensions were mixed with sense or antisense sRNAs and inoculated onto susceptible Arabidopsis seedlings. While sense sRNAs had no obvious effect on Hpa pathogenicity, antisense sRNAs inhibited spore germination and hence infection. Such inhibition of infection was not race‐specific, but dependent on the length and capping of sRNAs. Inhibition of infection by double stranded sRNA was more efficient than that observed with antisense sRNA. Thus, exogenous sRNA targeting conserved CesA3 could suppress Hpa infection in Arabidopsis, indicating the potential of this simple and efficient sRNA‐based approach for deciphering gene functions in obligate biotrophic pathogens as well as for R‐gene independent control of diseases in plants.

Item Type: Article
Additional Information:

The full-text of the online published article can be accessed via the official URL.

© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Uncontrolled Keywords: Arabidopsis, downy mildew, oomycetes, spray-induced gene silencing (SIGA), sRNA
Divisions: Divisions (2019 onwards) > College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:
Copyright Info: Open Access article (UW LS APC)
Depositing User: Mahmut Tor
Date Deposited: 03 Oct 2019 14:25
Last Modified: 05 Nov 2019 15:17
URI: https://eprints.worc.ac.uk/id/eprint/8693

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.