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Expression of Arabidopsis WEE1 in Tobacco Induces Unexpected Morphological and Developmental Changes

Siciliano, Ilario, Lentz Grønlund, A., Ševčíková, H., Spadafora, Natasha D., Rafiei, G., Francis, D., Herbert, Rob, Bitonti, M.B., Rogers, H.J. and Lipavská, H. (2019) Expression of Arabidopsis WEE1 in Tobacco Induces Unexpected Morphological and Developmental Changes. Scientific Reports, 9 (1). Article no. 8695. ISSN Online: 2045-2322

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Abstract

WEE1 regulates the cell cycle by inactivating cyclin dependent protein kinases (CDKs) via phosphorylation. In yeast and animal cells, CDC25 phosphatase dephosphorylates the CDK releasing cells into mitosis, but in plants, its role is less clear. Expression of fission yeast CDC25 (Spcdc25) in tobacco results in small cell size, premature flowering and increased shoot morphogenetic capacity in culture. When Arath;WEE1 is over-expressed in Arabidopsis, root apical meristem cell size increases, and morphogenetic capacity of cultured hypocotyls is reduced. However expression of Arath;WEE1 in tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants, and in BY2 cultures cell size was reduced. This phenotype is similar to expression of Spcdc25 and is consistent with a dominant negative effect on WEE1 action. Consistent with this putative mechanism, WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis. The phenotype is not due to sense-mediated silencing of WEE1, as overall levels of WEE1 transcript were not reduced in BY2 lines expressing Arath;WEE1. However the pattern of native WEE1 transcript accumulation through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native WEE1 transcription.

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2019

Uncontrolled Keywords: cell size, flowering, morphogenesis, plant cell cycle, tobacco BY2 cells, 49 transcriptional control, WEE1
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
Divisions: Academic Departments > Institute of Science and the Environment
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Copyright Info: Open access article
Depositing User: Rob Herbert
Date Deposited: 19 Jun 2019 10:12
Last Modified: 09 Jul 2019 12:07
URI: https://eprints.worc.ac.uk/id/eprint/8235

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