Filatov, V. and Dowdle, J. and Smirnoff, N. and Ford-Lloyd, B.V. and Newbury, John and Macnair, M.R. (2007) A Quantitative Trait Loci Analysis of Zinc Hyperaccumulation in Arabidopsis Halleri. New Phytologist, 174 (3). pp. 580-590.Full text not available from this repository. (Request a copy)
• The mechanisms of metal hyperaccumulation are still not understood, so we conducted a quantitative trait locus (QTL) analysis of zinc (Zn) hyperaccumulation in Arabidopsis halleri, in a cross between this and its sister species, A. petraea, in order to determine the number and approximate location of the genomic regions significantly contributing to this adaptation. • An F2 cross between the two species was made, and the leaf Zn concentration of 92 individuals was measured at both low (10 μM) and high (100 μM) Zn concentrations. Twenty-five markers were established that were distributed on all of the eight chromosomes. • Mapping of the markers established that they were essentially collinear with previous studies. QTLs exceeding a logarithm to the base 10 of the odds (LOD) value of 3 were found on chromosomes 4 (low Zn), 6 (high Zn) and 7 both high and low Zn). Evidence for a QTL on chromosome 3 (low Zn) was also found. • This analysis validates a previously used method of QTL analysis, based on microarray analysis of segregating families. Genes that have altered during the evolution of this character should also be QTL: this analysis calls into question a number of candidate genes from consideration as such primary genes because they do not appear to be associated with QTLs.
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|Uncontrolled Keywords:||Arabidopsis lyrata, Arabidopsis petraea, Arabidopsis halleri, hyperaccumulation, quantitative trait locus (QTL), zinc (Zn).|
|Subjects:||Q Science > QK Botany
Q Science > QD Chemistry
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Depositing User:||John Newbury|
|Date Deposited:||03 Dec 2009 15:38|
|Last Modified:||06 Aug 2015 11:46|
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