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Field Evaluation of a Competitive Lateral-flow Assay for Detection of Alternaria brassicae in Vegetable Brassica Crops

Wakeham, Alison and Keane, Gary and Kennedy, R. (2016) Field Evaluation of a Competitive Lateral-flow Assay for Detection of Alternaria brassicae in Vegetable Brassica Crops. Plant Disease, 100 (9). pp. 1831-1839. ISSN 0191-2917

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Abstract

On-site detection of inoculum of polycyclic plant pathogens could potentially contribute to management of disease outbreaks. A 6-min, in-field competitive immunochromatographic lateral flow device (CLFD) assay was developed for detection of Alternaria brassicae (the cause of dark leaf spot in brassica crops) in air sampled above the crop canopy. Visual recording of the test result by eye provides a detection threshold of approximately 50 dark leaf spot conidia. Assessment using a portable reader improved test sensitivity. In combination with a weather-driven infection model, CLFD assays were evaluated as part of an in-field risk assessment to identify periods when brassica crops were at risk from A. brassicae infection. The weather-driven model overpredicted A. brassicae infection. An automated 7-day multivial cyclone air sampler combined with a daily in-field CLFD assay detected A. brassicae conidia air samples from above the crops. Integration of information from an in-field detection system (CLFD) with weather-driven mathematical models predicting pathogen infection have the potential for use within disease management systems.

Item Type: Article
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The full-text is published as follows:-

Field Evaluation of a Competitive Lateral-Flow Assay for Detection of Alternaria brassicae in Vegetable Brassica Crops. Alison J. Wakeham, Gary Keane, and Roy Kennedy
Plant Disease, September 2016, Volume 100, Number 9 , Pages 1831-1839
http://dx.doi.org/10.1094/PDIS-10-15-1211-RE

Uncontrolled Keywords: disease management, evaluation, vegetable brassica crops
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Academic Departments > Institute of Science and the Environment
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Depositing User: Alison Wakeham
Date Deposited: 08 Sep 2016 15:08
Last Modified: 09 Sep 2016 08:15
URI: https://eprints.worc.ac.uk/id/eprint/4858

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