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Development of Molecular Markers Tightly Linked to Pvr4 Gene in Pepper Using Next-Generation Sequencing

Devran, Z. and Kahveci, E. and Özkaynak, E. and Studholme, D.J. and Tör, M. (2015) Development of Molecular Markers Tightly Linked to Pvr4 Gene in Pepper Using Next-Generation Sequencing. Molecular Breeding, 35 (101). ISSN Print: 1380-3743 Online: 1572-9788

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Abstract

It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site–leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.

Item Type: Article
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Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)

Uncontrolled Keywords: Potato virus Y, pepper, Pvr4, Nextgeneration, sequencing, synteny, MAS
Subjects: Q Science > Q Science (General)
Divisions: Academic Departments > Institute of Science and the Environment
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Copyright Info: Open Access article
Depositing User: Janet Davidson
Date Deposited: 24 Mar 2015 11:43
Last Modified: 18 Feb 2016 15:20
URI: https://eprints.worc.ac.uk/id/eprint/3647

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