Gonza´lez-Pe´rez, Lien and Va´zquez-Gları´a, Alenna and Perotta, Lara and Acosta, Alexis and Herbert, Rob and Cabrera, Juan Carlos and Francis, Dennis and Rogers, Hilary (2012) Oligosaccharins and Pectimorf(R) Stimulate Root Elongation and Shorten the Cell Cycle in Higher Plants. Plant Growth Regulation, 68 (2). pp. 211-221. ISSN 0167-6903 (print version) ISSN: 1573-5087 (electronic version)
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Official URL: http://www.springerlink.com/content/j228k7t5745272...
The aim was to test promotive effects of oligosaccharins on root growth and development at the root apical meristem and the cell cycle using the model systems,Arabidopsis thaliana and the tobacco (Nicotiana tabacum)BY-2 cell line. Arabidopsis was grown on medium supplemented with 0.1 mg L-1 oligoxyloglucan (OX), 10 mg L-1 Pectimorf (P) or 0.5 mg L-1 indole butyric acid (IBA). Primary root length, number of lateral root primordia, root apical meristem (RAM) length and epidermal cell length were recorded. Three genotypes were used: wild type (WT) and transgenic lines expressing either Schizosaccharomyces pombe (Sp) cdc25 or over-expressing(oe)Arath;WEE1. All treatments promoted primary root elongation but repressed lateral root production. Only P had a clear positive effect on meristem length whereas all other genotype 9 treatment interactions showed shorter RAMs. Whilst IBA, OX and P induced an increase in cell length in Spcdc25, the same treatments caused a significant decrease in WEE1oe. Mitotic indices were also significantly higher in roots treated with oligosaccharins suggesting a shortening of the cell cycle. This hypothesis was tested in the BY-2 cell line. Both OX and P shortened the cell cycle exclusively through a shortening of G1 whilst mitotic cell size remained constant between treatments. In conclusion, both OX and P do indeed stimulate growth and shorten the cell cycle in higher plants and at the cellular level are able to reverse large and small cell size phenotypes normally exhibited by WEE1oe and Spcdc25 genotypes, respectively.
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|Uncontrolled Keywords:||Arabidopsis thaliana BY-2 cells Cell cycle Lateral roots Nicotiana tabacum Oligosaccharins Plant growth regulators Root elongation Root morphology|
|Subjects:||Q Science > QK Botany|
Q Science > QH Natural history > QH301 Biology
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Deposited By:||Rob Herbert|
|Deposited On:||29 Oct 2012 18:13|
|Last Modified:||15 Nov 2012 12:15|
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