University of Worcester Worcester Research and Publications

Release of Bet v 1 From Birch Pollen From 5 European Countries. Results From the HIALINE Study

Buters, J.T.M. and Thibaudon, M. and Smith, Matthew and Kennedy, Roy and Rantio-Lehtimäki, A and Albertini, R and Reese, G and Weber, B and Carmen, G and Brandao, R and Antunes, C and Jaeger, S and Berger, U and Celenk, S and Grewling, L. and Bogdan, J and Sauliene, I and Weichenmeier, I and Pusch, G and Sarioglu, H and Ueffing, M and Behrendt, H and Prank, M and Sofiev, M and Cecchi, L (2012) Release of Bet v 1 From Birch Pollen From 5 European Countries. Results From the HIALINE Study. Atmospheric Environment, 55 . pp. 496-505. ISSN 1352-2310

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Exposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content of ambient air is unknown. We therefore monitored atmospheric concentrations of birch pollen grains and the matched major birch pollen allergen Bet v 1 simultaneously across Europe within the EU-funded project HIALINE (Health Impacts of Airborne Allergen Information Network). Pollen count was assessed with Hirst type pollen traps at 10 l min!1 at sites in France, United Kingdom, Germany, Italy and Finland. Allergen concentrations in ambient air were sampled at 800 l min!1 with a Chemvol! high-volume cascade impactor equipped with stages PM > 10 mm, 10 mm > PM > 2.5 mm, and in Germany also 2.5 mm > PM > 0.12 mm. The major birch pollen allergen Bet v 1 was determined with an allergen specific ELISA. Bet v 1 isoform patterns were analyzed by 2D-SDS-PAGE blots and mass spectrometric identification. Basophil activation was tested in an Fc 3R1-humanized rat basophil cell line passively sensitized with serum of a birch pollen symptomatic patient. Compared to 10 previous years, 2009 was a representative birch pollen season for all stations. About 90% of the allergen was found in the PM > 10 mm fraction at all stations. Bet v 1 isoforms pattern did not vary substantially neither during ripening of pollen nor between different geographical locations. The average European allergen release from birch pollen was 3.2 pg Bet v 1/pollen and did not vary much between the European countries. However, in all countries a >10-fold difference in daily allergen release per pollen was measured which could be explained by long-range transport of pollen with a deviating

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Uncontrolled Keywords: birch, pollen, Bet v 1, exposure, isoforms, ELISA, HIALINE, human, Basophils, Europe
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Research Centres > National Pollen and Aerobiology Research Unit
Depositing User: Sally Wall
Date Deposited: 14 May 2012 08:56
Last Modified: 15 Nov 2012 14:38

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