Wakeham, Alison and Kennedy, Roy (2010) Risk Assessment Methods for the Ringspot Pathogen Mycosphaerella Brassicicola in Vegetable Brassica Crops. Plant Disease, 94 (7). pp. 851-859. ISSN 0191-2917Full text not available from this repository. (Request a copy)
Mycosphaerella brassicicola causes ringspot on Brussels sprouts, which can result in substantial yield loss in commercial production. Brussels sprout buttons are downgraded if this pathogen occurs on them. In this study, the effect of temperature and wetness duration was investigated on infection of Brussels sprouts using controlled environments (CE). The effect of temperature and wetness duration on inoculum production and ascospore discharge was also investigated. Infection by M. brassicicola was described using a mathematical model and was compared to estimates of ascospore availability obtained via a volumetric air sampler and immunofluorescence (IF). Infection of M. brassicicola was correlated (r = 0.92) with temperature during leaf wetness periods. The relationship between temperature and time to discharge of 5 and 50% of the cumulative total number of ascospores from ringspot lesions was r = 0.99 and 0.98, respectively (P < 0.001). In field experiments, an optimal wind run (the product of the average wind speed and the period over which that average speed was measured) of 250 to 500 km day-1 was required for the dissemination of ringspot inoculum to field bait plants. Quantification of M. brassicicola inoculum in collected field aerosols was possible using a monoclonal antibody in a plate-trapped antigen enzyme-linked immunosorbent assay. Precoating of the air sampler wells with sodium azide prevented trapped spores from germinating. Ringspot inoculum could be detected and quantified in air samples from commercial crops of Brussels sprouts in the United Kingdom. Low levels of ringspot inoculum measured within crops did not lead to disease development.
The full-text of this article is available on Level 4 at The Hive in: Wakeham, Alison. The development and application of immunological tests within horticultural crop disease management systems. University of Worcester, PhD by Portfolio, 2014.
The full-text can be accessed via the official URL.
Originally deposited as National Pollen and Aerobiology Research Unit (NPARU)
|Uncontrolled Keywords:||plant disease, crop infection, ringspot, pathogens, risk assessment|
|Subjects:||S Agriculture > SB Plant culture|
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Copyright Info:||Open Access article|
|Depositing User:||Sally Wall|
|Date Deposited:||15 Mar 2012 10:22|
|Last Modified:||17 Feb 2016 16:20|
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