Rehmany, A.P. and Lynn, J.R. and Tör, M. and Holub, E.B. and Beynon, J.L. (2000) A Comparison of Peronospora parasitica (Downy mildew) Isolates from Arabidopsis thaliana and Brassica oleracea Using Amplified Fragment Length Polymorphism and Internal Transcribed Spacer 1 Sequence Analyses. Fungal Genetics and Biology, 30 (2). pp. 95-103.Full text not available from this repository. (Request a copy)
Amplified fragment length polymorphism (AFLP) fingerprints and internal transcribed spacer 1 (ITS1) sequences from 27 Peronospora parasitica isolates (collected from Arabidopsis thaliana or Brassica oleracea), 5 Albugo candida isolates (from the same hosts and from Capsella bursa-pastoris), and 1 Bremia lactucae isolate (from Lactuca sativa) were compared. The AFLP analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. The only exception was a group consisting of A. candida isolates from both B. oleracea and C. bursa-pastoris. ITS1 sequence analysis divided the isolates into the same five groups, demonstrated the divergence between P. parasitica isolates from A. thaliana and B. oleracea, and, using previously published ITS1 sequences, clearly showed the relationship between A. candida isolates from different hosts.
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Originally deposited as joint division with National Pollen and Aerobiology Research Unit (NPARU)
|Uncontrolled Keywords:||phylogenetic tree, Peronosporales, oomycete, Downey Mildew, Arabidopsis|
|Subjects:||Q Science > QK Botany
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
|Divisions:||Academic Departments > Institute of Science and the Environment|
|Depositing User:||Janet Davidson|
|Date Deposited:||19 Jan 2011 19:55|
|Last Modified:||12 Feb 2016 15:42|
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