University of Worcester Worcester Research and Publications
 
  USER PANEL:
  ABOUT THE COLLECTION:
  CONTACT DETAILS:

Genomic-Assisted Marker Development Suitable for CsCvy-1 Selection in Cucumber Breeding

Kahveci, E., Devran, Z., Özkaynak, E., Hong, Yiguo, Studholme, D.J. and Tör, M. ORCID: https://orcid.org/0000-0002-4416-5048 (2021) Genomic-Assisted Marker Development Suitable for CsCvy-1 Selection in Cucumber Breeding. Frontiers in Plant Science, 12 (691576). ISSN 1664-462X

[img]
Preview
Text
CsCvy-1 published.pdf - Published Version
Available under License Creative Commons Attribution.

Download (741kB) | Preview

Abstract

Cucumber is a widely grown vegetable crop plant and a host to many different plant pathogens. Cucumber vein yellowing virus (CVYV) causes economic losses on cucumber crops in Mediterranean countries and in some part of India such as West Bengal and in African countries such as Sudan. CVYV is an RNA potyvirus transmitted mechanically and by whitefly (Bemisia tabaci) in a semipersistent manner. Control of this virus is heavily dependent on the management of the insect vector and breeding virus-resistant lines. DNA markers have been used widely in conventional plant breeding programs via marker-assisted selection (MAS). However, very few resistance sources against CVYV in cucumber exist, and also the lack of tightly linked molecular markers to these sources restricts the rapid generation of resistant lines. In this work, we used genomics coupled with the bulked segregant analysis method and generated the MAS-friendly Kompetitive allele specific PCR (KASP) markers suitable for CsCvy-1 selection in cucumber breeding using a segregating F2 mapping population and commercial plant lines. Variant analysis was performed to generate single-nucleotide polymorphism (SNP)-based markers for mapping the population and genotyping the commercial lines. We fine-mapped the region by generating new markers down to 101 kb with eight genes. We provided SNP data for this interval, which could be useful for breeding programs and cloning the candidate genes.

Item Type: Article
Additional Information:

This article was submitted to Plant Breeding,
a section of the journal Frontiers in Plant Science.

A pdf file of this article is available to download from this WRaP record.

Copyright © 2021 Kahveci, Devran, Özkaynak, Hong, Studholme and Tör. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Uncontrolled Discrete Keywords: CVYV, cucumber, marker, assisted selection, Kompetitive allele-specific PCR genotyping, plant breeding
Divisions: College of Health, Life and Environmental Sciences > School of Science and the Environment
Related URLs:
Copyright Info: Open Access article
Depositing User: Mahmut Tor
Date Deposited: 24 Aug 2021 09:28
Last Modified: 24 Aug 2021 09:28
URI: https://eprints.worc.ac.uk/id/eprint/11293

Actions (login required)

View Item View Item
 
     
Worcester Research and Publications is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.